Rapid Discovery of the Potential Toxic Compounds in Polygonum multiflorum by UHPLC/Q-Orbitrap-MS-Based Metabolomics and Correlation Analysis

Conclusion Although there were several reports on the hepatotoxic compounds of PM, they were either evaluated by pure compounds or targeted analysis of different PM samples. Both of them are in lack of the integration of chemical constituents and toxic activities. In this study, we elaborated a metabolomics approach to analyze the variation of chemical constituents during the processing of PM, and integrated toxic activity evaluations. Both OPLS-DA analysis of the metabolites variations in post processing of PM and the correlation analysis between flow cytometry test and different metabolites in post processed PM enabled the identification of two toxic compounds. They were torachrysone-O-hexose (Comp. 83) and emodin-8-O-glucoside (Comp. 87) which both decreased significantly after processing. These two toxic compounds could be used as the potential toxic markers of PM. Further investigation of these two toxic compounds in the post processed samples suggested the best processing time was 24 h. Our experiments successfully validated two toxic compounds in PM, and provided enriched information for Chinese Pharmacopeia on the processing time of PM. Author Contributions LfH and PW performed the experiments and wrote the manuscript. QZ and FZ analyzed the data. LmH, CL, and YW guided the experiments. ZD, CL, WY, and YW revised the manuscript. LmH acquired funding for the research. All the authors read and approved the final manuscript. Funding This work was supported by Import...
Source: Frontiers in Pharmacology - Category: Drugs & Pharmacology Source Type: research