Comparative anti-inflammatory effects of pentoxifylline, polymyxin B, tyloxapol and flunixin on acute phase proteins, antioxidants and cardiovascular biomarkers during inflammatory responses in dromedary calves

Publication date: Available online 15 April 2019Source: Small Ruminant ResearchAuthor(s): Amir Saeed Samimi, Marzieh Karimiafshar, Kamyab SamimiAbstractThe present study aims to 1- assessment of acute phase proteins, antioxidants and cardiovascular biomarkers during inflammatory response which was induced by Escherichia coli (E. coli) lipopolysaccharide (LPS) in dromedary calves and 2- comparatively evaluate the anti-inflammatory effects of intravenous (IV) pentoxifylline, polymyxin B, tyloxapol and flunixin thereafter. A total of thirty male dromedary calves with the age of 20 ± 2 weeks and weight of 100 ± 5 kg. The calves were assigned randomly to five experimental groups (4 treatment and 1 positive control). Phenol extracted LPS at 0.1 μg kg-1 from E.coli serotype O55:B5 was used to induce inflammation. Six hours after induction of inflammatory responses, calves in groups Pntx, Plmx, Tlxp and Flnx IV received pentoxifylline (60 mg kg-1), polymyxin B (6000 U kg-1), tyloxapol (400 mg kg-1) and flunixin (2.2 mg kg-1), respectively. Calves in the positive control group were assigned to receive LPS without any anti-inflammatory drugs. Our results showed that values of heart rate (HR), respiratory rate (RR), rectal temperature (RT), packed cell volume (PCV), white blood cell (WBC), haptoglobin (HP), serum amyloid A (SAA), cardiac troponin I (cTnI) and hemocysteine (Hcy) were increased and glutathione peroxidase (GPx) and superoxide dismutase (SOD) we...
Source: Small Ruminant Research - Category: Zoology Source Type: research