Establishment and Application of Multiple Cross Displacement Amplification Coupled With Lateral Flow Biosensor (MCDA-LFB) for Visual and Rapid Detection of Candida albicans in Clinical Samples

In conclusion, we developed a MCDA-LFB assay for rapid and effective detection of C. albicans. The LOD of the new assay for C. albicans detection from pure cultures was as low as 200 fg. In comparison to other molecular methods (PCR, LAMP) for detection of C. albicans, the MCDA-LFB method is more sensitive, time-saving, and does not require expensive equipment or diagnostic technology. When using the MCDA-LFB assay to detect infected clinical samples, the results were consistent with those of the gold-standard method. This suggests the possibility for further clinical application of our C. albicans-MCDA-LFB assay. Ethics Statement This study was approved by the Ethics Committee of Shougang Hospital, and conducted according to the regulations of the Ministry of Health, China. The protocol was approved by the Ethics Committee of Shougang Hospital. Patients who provided sputum samples gave written informed consent, in accordance with the Declaration of Helsinki. Disclosure SH, FaZ, LN, LY, JN, FeZ, NG, XZ, LW, and CW have filed for a patent from the State Intellectual Property Office of the People's Republic of China, which includes the sequences and the MCDA-LFB method used for detecting C. albicans in this manuscript [application number CN 201811580513.9]. Author Contributions FaZ, JN, and SH conceived and designed the experiments; FaZ, LY, CW, JW, NG, XZ, and LW performed the experiments; FeZ, JN, LN, NG, XZ, and LW analyzed the data; FaZ, LN, FaZ, and SH contri...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research