The Human Upper Respiratory Tract Epithelium Is Susceptible to Flaviviruses

In this study, we exposed apically well-differentiated human NECs cultured at the ALI to the related flaviviruses ZIKV, JEV, WNV, and Usutu virus (USUV). We selected these viruses due to the recent increasing evidences of potential threat to humans (Cadar et al., 2017; Simonin et al., 2018). We show that NECs are particularly susceptible to JEV and WNV infection and to other flaviviruses included in this study. Infection with each virus led to shedding of infectious virus particles through the apical and basolateral surfaces and triggered host mechanisms at the level of inflammatory and antiviral mediators. Given the evidences of contact transmission in humans and detection of flaviviruses RNA in upper respiratory tract samples, our data suggest that the human respiratory epithelium could represent a target for the replication of flaviviruses. Materials and Methods Virus Propagation JEV (G1; CNS769_Laos_2009, GenBank accession number KC196115.1) was kindly provided by R. Charrel Aix-Marseille Université, Marseille, France. WNV (NY99–35, GenBank accession number DQ211652.1) and USUV (SAAR-1776, GenBank accession number AY453412) were kindly provided by R. Hoop, Institute of Veterinary Bacteriology, University of Zurich, Zurich, Switzerland. The low passage clinical isolate of Asian lineage ZIKV (PRVABC59) was obtained at PHE (Public Health England). All flaviviruses were passaged on Vero cells cultured in DMEM (Gibco) supplemented with 10% FBS (Bio...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research

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DISCUSSION: Our data does not appear to suggest a significant protective effect of smoking on the occurrence of KC. It shows a lesser proportion of smokers in KC patients after A-CXL, but this difference did not exist at the time of KC diagnosis. PMID: 31204085 [PubMed - as supplied by publisher]
Source: Journal Francais d Ophtalmologie - Category: Opthalmology Tags: J Fr Ophtalmol Source Type: research
Mai Cuisine Inc., of Philadelphia, PA is voluntarily recalling 31 packs of Spicy Shrimp Tempura Roll and 32 packs of Spicy Shrimp Tempura Crunch Roll because it may contain undeclared egg.
Source: FDA Food Alergies - Category: Food Science Authors: Source Type: alerts
Authors: Kelkay B, Omer A, Teferi Y, Moges Y Abstract Background: Preterm birth is the leading cause of neonatal mortality and significant health consequences to the newborn, families, and communities and tens of emotional and economic costs. Therefore, the aim of this study was to assess the magnitude of singleton preterm birth and associated factors in Shire Suhul General Hospital, Northern Ethiopia. Methods: Institutional based cross-sectional study was conducted among systematically selected 325 postnatal mothers in Shire Suhul General Hospital, Northern Ethiopia, from January to March 2018. The data were c...
Source: Journal of Pregnancy - Category: OBGYN Tags: J Pregnancy Source Type: research
In this study, we investigated the adequacy of pharmacists' instructions on the amount of topical steroids to be applied and the way in which they enhance the effect of pharmaceutical interventions. A self- administered anonymous questionnaire was distributed using QLifePro to 300 pharmacists working in insurance pharmacies that filled at least one dermatologist's prescription per month on average in Japan. Out of 300 pharmacists, 196 (65.3%) recognized the Japanese Dermatological Association's 2016 guidelines for the treatment AD, 107 (35.6%) gave instructions using the FTU as an index of external dose of topical steroids...
Source: Drug Discoveries and Therapeutics - Category: Drugs & Pharmacology Tags: Drug Discov Ther Source Type: research
In this study, a multipurpose integrated syringe-pump-based environmental-water analyzer (iSEA) was combined with an on-line filtration system for underway analysis of nitrate and nitrite in saline samples. Vanadium chloride was used instead of a toxic cadmium column to reduce nitrate to nitrite, which was measured on the basis of the classic Griess reaction. This fully automated analyzer had a limit of detection of 0.02 μmol L-1 for nitrite and 0.14 μmol L-1 for nitrate. The sample throughput was 12 h-1 for simultaneous measurement of nitrite and nitrate. With automated dilution, the calibration curve for ...
Source: Analytica Chimica Acta - Category: Chemistry Authors: Tags: Anal Chim Acta Source Type: research
Chem. Commun., 2019, Accepted Manuscript DOI: 10.1039/C9CC04239C, CommunicationDongyang Lou, Congsen Wang, Zhiyong He, Xiaoyi Sun, Jiasheng Luo, Juan Li A tough double-network (DN) organohydrogel, obtained by simply soaking the PAMPS/PAAm hydrogel in ethylene glycol solution of lithium chloride, retains high mechanical performance, flexibility ( −80 ~ 120 oC) and conductivity... The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Chem. Commun. latest articles - Category: Chemistry Authors: Source Type: research
Publication date: Available online 18 June 2019Source: Advances in Space ResearchAuthor(s): Leonardo Marini-Pereira, Kelias de Oliveira, Lucas A. Salles, Alison de O. Moraes, Eurico R. de Paula, Marcio Tadeu de Assis Honorato Muella, Waldecir J. PerrellaAbstractThe ionosphere in low-latitude regions has intense dynamics with great variability – not only spatially but also temporally. The most critical effect found in the low-latitude region is the scintillation in phase and amplitude due to plasma bubble occurrence in the ionospheric layer. To augmentation systems like GBAS or SBAS, designed to provide Category-I (CA...
Source: Advances in Space Research - Category: Science Source Type: research
[Deutsche Welle] Declaring a global health emergency could galvanize aid to fight the Ebola epidemic in the DR Congo. It could also make the job of containing the virus much more difficult. The WHO's decision proved controversial.
Source: AllAfrica News: Health and Medicine - Category: African Health Source Type: news
We examined fifteen years of cross-sectional data (2002–2016, N = 234,803) from the National Survey on Drug Use and Health. Our main outcome was youth self-reported (no/yes) past-year participation in a pregnancy or STI prevention program. Survey adjusted prevalence estimates and logistic regression analysis were used to examine trends in participation. Youth participation in pregnancy and STI prevention programming decreased significantly from a high of 15% in 2003 to a low of 7% in 2016. Representing a 53% proportional decline in youth participation, this downward trend was significant even when controlling for...
Source: Preventive Medicine - Category: International Medicine & Public Health Source Type: research
Publication date: Available online 18 June 2019Source: Preventive MedicineAuthor(s): Benjamin D. Hallowell, Meheret Endeshaw, Matthew T. McKenna, Virginia Senkomago, Hilda Razzaghi, Mona SaraiyaAbstractFrom 1970 to 2010 the foreign-born population in the United States has rapidly increased from 9.6 to 40.0 million individuals. Historically, differences in cancer rates have been observed between US-born and foreign-born individuals. However, comprehensive and up-to-date data on US cancer rates by birth place is lacking. To compare cancer mortality rates among foreign and US-born individuals, population-based cancer mortalit...
Source: Preventive Medicine - Category: International Medicine & Public Health Source Type: research
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