The Human Upper Respiratory Tract Epithelium Is Susceptible to Flaviviruses

In this study, we exposed apically well-differentiated human NECs cultured at the ALI to the related flaviviruses ZIKV, JEV, WNV, and Usutu virus (USUV). We selected these viruses due to the recent increasing evidences of potential threat to humans (Cadar et al., 2017; Simonin et al., 2018). We show that NECs are particularly susceptible to JEV and WNV infection and to other flaviviruses included in this study. Infection with each virus led to shedding of infectious virus particles through the apical and basolateral surfaces and triggered host mechanisms at the level of inflammatory and antiviral mediators. Given the evidences of contact transmission in humans and detection of flaviviruses RNA in upper respiratory tract samples, our data suggest that the human respiratory epithelium could represent a target for the replication of flaviviruses. Materials and Methods Virus Propagation JEV (G1; CNS769_Laos_2009, GenBank accession number KC196115.1) was kindly provided by R. Charrel Aix-Marseille Université, Marseille, France. WNV (NY99–35, GenBank accession number DQ211652.1) and USUV (SAAR-1776, GenBank accession number AY453412) were kindly provided by R. Hoop, Institute of Veterinary Bacteriology, University of Zurich, Zurich, Switzerland. The low passage clinical isolate of Asian lineage ZIKV (PRVABC59) was obtained at PHE (Public Health England). All flaviviruses were passaged on Vero cells cultured in DMEM (Gibco) supplemented with 10% FBS (Bio...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research

Related Links:

The confidence of intimate partner violence (IPV) survivors/victims in the criminal justice system (CJS) is important to consider when exploring intervention and prevention strategies toward deterring IPV. Information on the experiences of IPV survivors/vi...
Source: SafetyLit - Category: International Medicine & Public Health Tags: Jurisprudence, Laws, Legislation, Policies, Rules Source Type: news
Publication date: Available online 8 July 2020Source: Case Reports in Women's HealthAuthor(s): Zuzana Kolkova, Martin F. Bjurström, John-Kalle Länsberg, Eimantas Svedas, Maria Andrada Hamer, Stefan R. Hansson, Andreas Herbst, Mehreen Zaigham
Source: Case Reports in Womens Health - Category: OBGYN Source Type: research
CBSN political reporter Caitlin Huey-Burns and Washington Post politics reporter Eugene Scott join CBSN's "Red&Blue" to discuss  the White House response to the coronavirus pandemic, plans for the Republican National Convention, and Joe Biden's presidential campaign.
Source: Health News: CBSNews.com - Category: Consumer Health News Source Type: news
As Indonesia’s death toll from the coronavirus continues to rise, the world’s most populous Muslim country finds itself at odds with protocols put in place by the government to handle the bodies of victims of the pandemic
Source: ABC News: Health - Category: Consumer Health News Tags: Health Source Type: news
Publication date: Available online 7 July 2020Source: SeizureAuthor(s): Yuval Shafrir
Source: Seizure - Category: Neurology Source Type: research
Source: BMJ Comments - Category: General Medicine Source Type: forums
Source: BMJ Comments - Category: General Medicine Source Type: forums
Authors: Pisché G, Spitz I, Siffray-Bauer L, Dentel C, Perriard J, Carré S PMID: 32631679 [PubMed - as supplied by publisher]
Source: Revue Neurologique - Category: Neurology Tags: Rev Neurol (Paris) Source Type: research
Source: Reuters: Health - Category: Consumer Health News Source Type: news
Source: Reuters: Health - Category: Consumer Health News Source Type: news
More News: Academia | African Health | Allergy | Allergy & Immunology | Asthma | Australia Health | Azithromycin | Biology | Blood Transusion | Brain | Brazil Health | Breastfed | Centers for Disease Control and Prevention (CDC) | Children | Chloride | Chronic Obstructive Pulmonary | Coccidioidomycosis (Valley Fever) | Cystic Fibrosis | Cytology | Dengue Fever | Encephalitis | Endemics | Epidemics | Epidemiology | France Health | Funding | Genetics | Germany Health | Grants | Guinea Health | H1N1 | Health Management | Indonesia Health | Infectious Diseases | Influenza | Influenza Vaccine | Intensive Care | International Medicine & Public Health | Japan Health | Laboratory Medicine | Laos Health | Men | Microbiology | Micronesia Health | Middle East Health | Milk | Molecular Biology | Nepal Health | Netherlands Health | Neurology | Outbreaks | Paraguay Health | Pediatrics | Perinatology & Neonatology | Pregnancy | Pulmonary Function Testing | Reproduction Medicine | Respiratory Medicine | Statistics | Study | Swine Flu | Swine Flu (H1N1) Vaccine | Switzerland Health | Turkey Health | USA Health | Vaccines | Venezuela Health | Veterinary Research | Veterinary Vaccinations | Virology | Vitamin D | Vitamins | West Nile Virus | Women | Zika Virus | Zithromax | Zoonoses