A Rapid Antimicrobial Susceptibility Test for Determining Yersinia pestis Susceptibility to Doxycycline by RT-PCR Quantification of RNA Markers

This study was supported by the Israel Institute for Biological Research, Ness Ziona, Israel. Conflict of Interest Statement The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. Acknowledgments We thank Dr. Vered Caspi (Ben-Gurion University) for assisting with the bioinformatics and statistical analyses of the Agilent DNA microarray results. Supplementary Material The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmicb.2019.00754/full#supplementary-material Footnotes ^ https://www.selectagents.gov/selectagentsandtoxinslist.html ^ https://afro.who.int/health-topics/plague/plague-outbreak-situation-reports ^ https://www.cdc.gov/plague/healthcare/clinicians.html ^ https://www.fda.gov/MedicalDevices/DeviceRegulationandGuidance/GuidanceDocuments/ucm080564.htm References Aloni-Grinstein, R., Schuster, O., Yitzhaki, S., Aftalion, M., Maoz, S., Steinberger-Levy, I., et al. (2017). Isolation of Francisella tularensis and Yersinia pestis from blood cultures by plasma purification and immunomagnetic separation accelerates antibiotic susceptibility determination. Front. Microbiol. 8:312. doi: 10.3389/fmicb.2017.00312 PubMed Abstract | CrossRef Full Text | Google Scholar Aloni-Grinstein, R., Shifman, O., Lazar, S., Steinberger-Levy, I., Maoz, S., and Ber, R. (2015). A rapid real-time...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research

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Chem. Commun., 2019, Advance Article DOI: 10.1039/C9CC02941A, CommunicationJinshuo Zou, Germanas Peleckis, Chong-Yong Lee, Gordon G. Wallace Ultrathin iron oxyhydroxide nanosheets were synthesised by multiple potential cyclings on thermally annealed iron foils, acting as an efficient oxygen evolution electrocatalyst. To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Chem. Commun. latest articles - Category: Chemistry Authors: Source Type: research
We report good clinical outcomes postoperative, with intact range of motion and no surgical complication at the 6-month follow-up visit. The patient has ongoing physical therapy to reduce any residual strength deficits.
Source: Techniques in Hand and Upper Extremity Surgery - Category: Surgery Tags: Techniques Source Type: research
Ulnar-sided carpometacarpal (CMC) joint fracture dislocations are relatively uncommon, frequently associated with fractures of the metacarpal base and/or hamate, and often sustained by young male individuals secondary to striking a hard object. The complexity of ulnar-sided CMC fracture dislocations necessitates careful restitution of anatomic alignment and joint congruency for mobility and stability. Miniaturization of the external fixation device has allowed application to the hand. Spanning external fixation utilizes the principles of “ligamentotaxis”—indirect reduction through distraction forces of ca...
Source: Techniques in Hand and Upper Extremity Surgery - Category: Surgery Tags: Techniques Source Type: research
We describe our approach as a “Link” between the most common approaches that surgeons are familiar with; the dorsoulnar approach to the thumb metacarpophalangeal joint for ulnar collateral ligament repair and the flexor carpi radialis approach for distal radial fracture fixation. The zone between these two incisions is not as frequently approached. Our incision connects these two well established incisions and we have described a step by step approach to this unfamiliar area. This “Link zone” overlies the thumb trapeziometacarpal joint, scaphotrapeziotrapezoid joint, and the radial styloid. It conta...
Source: Techniques in Hand and Upper Extremity Surgery - Category: Surgery Tags: Techniques Source Type: research
CONCLUSIONS: Current literature show similar risks for bacterial pathogens. Studies are limited for viral and parasitic pathogens. The evaluated risk-factors for PI-IBS varied among the included studies and the existing evidence is insufficient to identify pathogen-specific risk factors. PMID: 31112663 [PubMed - as supplied by publisher]
Source: Scandinavian Journal of Gastroenterology - Category: Gastroenterology Tags: Scand J Gastroenterol Source Type: research
Publication date: Available online 21 May 2019Source: Journal of Environmental PsychologyAuthor(s): Emmy van Esch, Robert Minjock, Stephen M. Colarelli, Steven HirschAbstractA growing number of organizations are re-designing workplaces to give employees greater exposure to natural views, which frequently improve well-being. But what is it about views of nature that produce these benefits? Is it the overall view or the particular features within a view? If it is features, what features have the greatest effects on well-being, and are these features particular to natural settings or might they generalize to built settings? W...
Source: Journal of Environmental Psychology - Category: Psychiatry & Psychology Source Type: research
Surgical treatment remains the standard of care for active patients with acute midsubstance Achilles tendon ruptures. Minimal invasive surgery, rupture’s site hematoma preservation, strong fixation, avoidance of sural nerve entrapment, and early rehabilitation are essential concepts desirable for any Achilles tendon repair. Hereby, we present a technique modification that incorporates all these advantages in one single procedure.
Source: Techniques in Foot and Ankle Surgery - Category: Orthopaedics Tags: Special Focus: Innovations in Foot and Ankle Sports Injury Treatment Source Type: research
The most reliable reconstruction for chronic ankle ligament instability continues to be debated. The conventional Broström is widely used, but there are limitations to the technique. By virtue of the tissue repair a conservative rehabilitation approach is advocated, and there is an increasing concern that the repair does not match the strength of the native ligament. That begs the question of whether an augmentation is needed and if it is done, how does it affect the native ligament repair and how does it stand up over time? This chapter will discuss these questions as well as our current approach to lateral ligament reconstruction.
Source: Techniques in Foot and Ankle Surgery - Category: Orthopaedics Tags: Special Focus: Innovations in Foot and Ankle Sports Injury Treatment Source Type: research
Ankle sprains are one of the most common injuries in foot and ankle, and the superior fascicle of the anterior talofibular ligament (ATFL) frequently is injured as a consequence. Patients with isolated rupture of ATFL’s superior fascicle can sustain a subtle ankle instability or ankle microinstability. Patients with symptomatic ankle microinstability may describe a subjective feeling of ankle instability, recurrent symptomatic ankle sprains, chronic anterolateral pain or a combination of any of them. When conservative management fails, anatomic all-inside ATFL’s superior fascicle repair under direct arthroscopi...
Source: Techniques in Foot and Ankle Surgery - Category: Orthopaedics Tags: Special Focus: Innovations in Foot and Ankle Sports Injury Treatment Source Type: research
Reported cases of mumps and pertussis have risen in the US in recent years even though many people are vaccinated. As part of the FDA commitment to protect the public health, FDA scientists are working to learn why more cases are occurring.
Source: FDA Consumer Health Information Updates - Category: Consumer Health News Authors: Source Type: news
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