Phenotypic Plasticity of Staphylococcus aureus in Liquid Medium Containing Vancomycin

Conclusion We chose vancomycin as an environmental stress factor to study the phenotypic plasticity of S. aureus. A bivariate GWAS was used to identify potential SNPs and genes responsible for growth plasticity. Our results provide an alternative strategy to dissect the adaptive growth of S. aureus in an antibiotic environment and highlight the feasibility of bivariate GWAS in the phenotypic plasticity research of bacteria. Author Contributions YJ and XH conceived and designed the experiments. MR, XZ, and JB performed the experiments. MY, XX, and YJ analyzed the data. MR and YJ wrote the manuscript. All authors reviewed the manuscript. Funding This work was supported by the Fundamental Research Funds for the Central University (2017JC05), Beijing Municipal Funds for Talent Training (2017000020124G276), National Forestry and Grassland Administration (2015[54]), and Science and Technology Service Network Initiative (KFJ-STS-ZDTP-036). Conflict of Interest Statement The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. Supplementary Material The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmicb.2019.00809/full#supplementary-material FIGURE S1 | Growth curves of 41 strains. The growth curves of all strains at two treatments. FIGURE S2 | Manhattan plots of significant SNPs. T...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research