Surface (S) Layer Proteins of Lactobacillus acidophilus Block Virus Infection via DC-SIGN Interaction
We describe the unexpected binding of S-layer to cells devoid of DC-SIGN but also confirm that the presence of DC-SIGN was essential for S-layer’s antiviral activity. S-layer protein exerted its antiviral effect with different kinetics than mannan, a known viral inhibitor that also acts on DC-SIGN (Yu et al., 2017). Together our results suggest that inhibition of viral entry by S-layer occurs via a novel S-layer/DC-SIGN interaction. Materials and Methods Isolation of S-Layer Proteins S-layer proteins were extracted from overnight cultures of L. acidophilus ATCC 4356 cells grown in MRS medium at 37°C by using 6 M LiCl. The protein was extensively dialyzed against distilled water overnight at 4°C and after centrifugation (10,000 × g 20 min), it was suspended in sterile H2O and stored at 20°C (Beveridge et al., 1997). Purity was evaluated by SDS-PAGE, which showed a single band after Coomassie blue staining. Cell Lines and Viruses Vero cells, 3T3 cells, and 3T3 cells stably expressing human DC-SIGN (3T3 DC-SIGN) were cultured at 37°C in Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum, 100 U penicillin/ml, and 100 μg streptomycin/ml. 3T3 parental and 3T3 DC-SIGN-expressing cells were a kind gift from Vineet N. Kewal Ramani, HIV Drug Resistance Program, NCI. SFV was a well-characterized plaque-purified isolate (Glomb-Reinmund and Kielian, 1998), CHIKV was the vaccine strain 181/25, obtained from Dr. Robert Tesh (...
Authors: Takada S, Saito MK, Kambe N Abstract Blau syndrome, or early-onset sarcoidosis, is hereditary juvenile-onset systemic granulomatosis. Clinical symptoms appear before the age of 4 years and mainly affect the skin, joints, and eyes. The symptoms are progressive and cause severe complications, such as joint destruction and blindness. Although tumor necrosis factor alpha (TNFα) antagonists are effective for controlling some of the symptoms of Blau syndrome, there is no specific curative treatment. Heterozygous mutations in nucleotide-binding oligomerization domain 2 (NOD2) were identified as the cause of...
Conclusion. A combination of chemotherapy and MSC can yield to more favorable results in the treatment of HCC. PMID: 32617114 [PubMed]
Authors: Zhan LH, Dong YJ, Yang K, Lei SS, Li B, Teng X, Zhou C, Luo R, Yu QX, Jin HY, Lv GY, Chen SH Abstract Suanzaoren decoction (SZRT), a classic Chinese herbal prescription, has been used as a treatment for insomnia for more than a thousand years. However, recent studies have found no significant effects of SZRT as a treatment for insomnia caused by gastric discomfort. Herein, we studied the effects of modified Suanzaoren decoction (MSZRD) on gastrointestinal disorder-related insomnia. The main constituents of MSZRD were spinosin (2.21 mg/g) and 6-feruloylspinosin (0.78 mg/g). A pentobarbital-ind...
Isolation of Oxyberberine and β-Sitosterol from Berberis lycium Royle Root Bark Extract and In Vitro Cytotoxicity against Liver and Lung Cancer Cell Lines. Evid Based Complement Alternat Med. 2020;2020:2596082 Authors: Anwar MA, Tabassam S, Gulfraz M, Sheeraz Ahmad M, Raja GK, Arshad M Abstract Berberis lycium Royle has been traditionally used to cure rheumatism, eye and ear diseases, malarial fever, diabetes, stomach disorders, and skin diseases. There is a least amount of data available on cytotoxic capacity of Berberis lycium from Pakistani origin, so on this basis, the present study was aimed ...
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Publication date: Available online 3 July 2020Source: The Journal of Chemical ThermodynamicsAuthor(s): Svetlana Blokhina, Marina Ol'khovich, Angelica Sharapova, German Perlovich
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