Surface (S) Layer Proteins of Lactobacillus acidophilus Block Virus Infection via DC-SIGN Interaction

We describe the unexpected binding of S-layer to cells devoid of DC-SIGN but also confirm that the presence of DC-SIGN was essential for S-layer’s antiviral activity. S-layer protein exerted its antiviral effect with different kinetics than mannan, a known viral inhibitor that also acts on DC-SIGN (Yu et al., 2017). Together our results suggest that inhibition of viral entry by S-layer occurs via a novel S-layer/DC-SIGN interaction. Materials and Methods Isolation of S-Layer Proteins S-layer proteins were extracted from overnight cultures of L. acidophilus ATCC 4356 cells grown in MRS medium at 37°C by using 6 M LiCl. The protein was extensively dialyzed against distilled water overnight at 4°C and after centrifugation (10,000 × g 20 min), it was suspended in sterile H2O and stored at 20°C (Beveridge et al., 1997). Purity was evaluated by SDS-PAGE, which showed a single band after Coomassie blue staining. Cell Lines and Viruses Vero cells, 3T3 cells, and 3T3 cells stably expressing human DC-SIGN (3T3 DC-SIGN) were cultured at 37°C in Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum, 100 U penicillin/ml, and 100 μg streptomycin/ml. 3T3 parental and 3T3 DC-SIGN-expressing cells were a kind gift from Vineet N. Kewal Ramani, HIV Drug Resistance Program, NCI. SFV was a well-characterized plaque-purified isolate (Glomb-Reinmund and Kielian, 1998), CHIKV was the vaccine strain 181/25, obtained from Dr. Robert Tesh (...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research

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Publication date: 2019Source: Methods in Microbiology, Volume 46Author(s): Surendra Gulla, Dakshayani Lomada, Vadali V.S.S. Srikanth, Muthukonda Venkatakrishnan Shankar, Kakarla Raghava Reddy, Sarvesh Soni, Madhava C. ReddyAbstractNanoparticles' based cancer therapeutics are greatly dependent on the development of science and engineering of nanoparticles. In this article, important strategies based on nanoparticles used for cancer treatment are discussed. The inspiration to write this article is not only to review the existing strategies based on nanoparticles but also to highlight the pertinent and possible solutions ther...
Source: Methods in Microbiology - Category: Microbiology Source Type: research
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Source: Methods in Microbiology - Category: Microbiology Source Type: research
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Source: Methods in Microbiology - Category: Microbiology Source Type: research
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Source: Methods in Microbiology - Category: Microbiology Source Type: research
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Source: Comptes Rendus Physique - Category: Physics Source Type: research
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Source: Biocatalysis and Agricultural Biotechnology - Category: Biotechnology Source Type: research
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Source: Canadian Journal of Ophthalmology - Category: Opthalmology Authors: Tags: Can J Ophthalmol Source Type: research
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Source: Canadian Journal of Ophthalmology - Category: Opthalmology Authors: Tags: Can J Ophthalmol Source Type: research
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Source: Molecular Phylogenetics and Evolution - Category: Molecular Biology Source Type: research
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