A Novel Antiserum Against a Predicted Human Peripheral Choline Acetyltransferase (hpChAT) for Labeling Neuronal Structures in Human Colon

This study was carried out in accordance with the recommendations of “NIH Guide for Care and Use of Laboratory Animal protocols” and of the Institutional Animal Care and Use Committee of Shiga University of Medical Science (SUMS). The protocol was approved by SUMS. Six adult male Balb/c mice (5-weeks-old) were purchased from Clea Japan Inc. (Konan, Japan). Each mouse (labeled H1-H6) received subcutaneous injections of 0.1 ml of TiterMax Gold adjuvant (Sigma-Aldrich, Saint-Louis, MO, USA) containing 5 mg/ml KLH-conjugated peptide into multiple sites of the neck and back skin with at a 2-week interval. Antisera, collected 4 days after immunization or booster injections were tested by IHC on sections of human colon. After 9–13 booster injections, the whole blood was collected from the heart under deep anesthesia with an intraperitoneal injection of sodium pentobarbital (80 mg/kg, Somnopentyl, Kyoritsu Seiyaku, Tokyo, Japan). After exsanguination, animals were euthanized by intracardiac injection with a lethal dose of sodium pentobarbital (150 mg/kg). The antiserum was isolated from collected mouse blood using a blood-serum separation device (Nipro, Osaka, Japan). All mice immunized gave high-titer antisera capable of labeling positive structures in human colon by IHC (Supplementary Figure S1). Among them, the mouse H3 gave the highest titer antiserum after 13 booster injections. Then, this H3 antiserum, termed here hpChAT antiserum, has been used in the present...
Source: Frontiers in Neuroanatomy - Category: Neurology Source Type: research

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ConclusionIn the future, the survey is to be converted to a mobile accessible application and extended to the entire Paralympic Team.Level of evidence3
Source: Sports Orthopaedics and Traumatology - Category: Sports Medicine Source Type: research
This study constituted the second phase of a World Anti-Doping Agency (WADA) supported Social Science initiative.
Source: Performance Enhancement and Health - Category: Sports Medicine Source Type: research
Publication date: October 2019Source: Social Networks, Volume 59Author(s): Hazem Krichene, Yoshi Fujiwara, Abhijit Chakraborty, Yoshiyuki Arata, Hiroyasu Inoue, Masaaki TeraiAbstractThis paper investigates the mechanisms behind the formation of links between suppliers and customers in the Tokyo Stock Exchange (TSE) production network. The supplier-customer network of listed firms in Japan is estimated by the so-called exponential random graph model (ERGM). We estimate 4 models to understand how endogenous and exogenous attributes contribute to the emergence of the TSE production network. In terms of endogenous attributes, ...
Source: Social Networks - Category: Psychiatry & Psychology Source Type: research
This study examines the relationships between pornography preferences, individual's sex, proxies for short-term mating strategies (e.g., life history strategy, sociosexuality), and infidelity. For example, we predict that men, more than women, will be interested in group sex (i.e., threesomes and gangbang) scenarios in pornography as a result of selective pressures for sperm competition. Further, specific activities and sexual situations in pornography are likely to be differentially appealing based on the sexual strategies pursued by consumers. Thus, women who have higher, versus lower, intentions of committing infidelity...
Source: Personality and Individual Differences - Category: Psychiatry & Psychology Source Type: research
Publication date: Available online 25 May 2019Source: Leukemia Research ReportsAuthor(s): Aya Nakaya, Shinya Fujita, Atsushi Satake, Takahisa Nakanishi, Yoshiko Azuma, Yukie Tsubokura, Ryo Saito, Akiko Konishi, Masaaki Hotta, Hideaki Yoshimura, Kazuyoshi Ishii, Tomoki Ito, Shosaku Nomura
Source: Leukemia Research Reports - Category: Hematology Source Type: research
Publication date: Available online 25 May 2019Source: American Heart JournalAuthor(s): Alexander C. Fanaroff, Amit N. Vora, Anita Y. Chen, Robin Mathews, Jacob A. Udell, Matthew T. Roe, Laine Thomas, Tracy Y. WangAbstractBackgroundLittle is known about the proportion of hospitals in the United States that offer clinical trial enrollment opportunities and how patient outcomes differ between hospitals that do and do not participate in clinical trials.MethodsIn the nationwide Chest Pain - MI registry, we described the proportion of hospitals that enrolled patients with acute myocardial infarction (MI) in clinical trials from ...
Source: American Heart Journal - Category: Cardiology Source Type: research
In this study, we found that cofilin competes with tau for direct microtubule binding in vitro, in cells, and in vivo, which inhibits tau-induced microtubule assembly. Genetic reduction of cofilin mitigates tauopathy and synaptic defects in Tau-P301S mice and movement deficits in tau transgenic C. elegans. The pathogenic effects of cofilin are selectively mediated by activated cofilin, as active but not inactive cofilin selectively interacts with tubulin, destabilizes microtubules, and promotes tauopathy. These results therefore indicate that activated cofilin plays an essential intermediary role in neurotoxic signaling th...
Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
Publication date: Available online 25 May 2019Source: Journal of Materials Research and TechnologyAuthor(s): Hassan Shokry HassanAbstractNano-zirconium tungsto-vanadate cation exchange material was constructed in different morphological structures using sol–gel, hydrothermal and microwave techniques in presence of polymeric matrix as stabilizing agent. Polyvinyl alcohol was established as the optimum stabilizing agent. Influence of preparation parameters in presence of polyvinyl alcohol was examined as a function of ion exchange capacity and surface area for the prepared materials. The optimum reactant molar ratio wa...
Source: Journal of Materials Research and Technology - Category: Materials Science Source Type: research
Publication date: Available online 25 May 2019Source: Journal of Materials Research and TechnologyAuthor(s): Yassine Ennaciri, Hanan El Alaoui-Belghiti, Mohammed BettachAbstractThis paper proposes a very attractive process for K2SO4 production via the wet conversion of phosphogypsum (PG) and K2CO3. A parallel study of the conversion of two synthetic types of gypsum by K2SO4 was performed in order to compare their reactivity with that of PG.We remark that PG is more reactive and yields the desired results while commercial types of gypsum react slowly and generate unwanted secondary products. The factors affecting the PG con...
Source: Journal of Materials Research and Technology - Category: Materials Science Source Type: research
Publication date: Available online 25 May 2019Source: Journal of Materials Research and TechnologyAuthor(s): Adawiya J. Haider, Riyad Al- Anbari, Hiba M. Sami, Mohammed J. HaiderAbstractIn this work, synthesis of nickel oxide (NiO) using nickel acetate as a starting material by sol–gel method and then deposited as collide on glass slides to form thin layers of NiO by spin-coating technique with high photo-catalytic activity using as self cleaning coating. These films of NiO were annealed at different temperature 450 °C, 550 °C. The structural, morphological, spectral and optical properties of prepar...
Source: Journal of Materials Research and Technology - Category: Materials Science Source Type: research
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