Expression of the Neuroblastoma-Associated ALK-F1174L Activating Mutation During Embryogenesis Impairs the Differentiation of Neural Crest Progenitors in Sympathetic Ganglia

In this study, we investigated the impact of the human ALK-F1174L mutation in vivo on sympathetic neuroblast differentiation using Sox10-Cre;LSL-ALK-F1174L mice. The aim was to improve our understanding of the implication of ALK deregulated signaling in the initial steps of NB genesis. Results ALK-F1174L Causes Embryonic Lethality When Expressed in NCCs Prior to SA Lineage Commitment To assess the impact of the human ALK-F1174L activating mutation on the development and differentiation of the SNS, Sox10-Cre;LSL-ALK-F1174L embryos were generated. This model allowed to restrict the expression of the ALK-F1174L variant to migrating Sox10+ cells before sympathetic lineage commitment. We observed that such lineage-specific expression of ALK-F1174L mediated an embryonic lethality in 100% of E12.5 Sox10-Cre;LSL-ALK-F1174L embryos (n = 4) (Figure 1). A similar phenotype was also observed in 1/8 and 1/3 Sox10-Cre;LSL-ALK-F1174L embryos at E10.5 and E11.5, respectively (data not shown). FIGURE 1 Figure 1. ALK-F1174L expression in Sox10+ cells NCCs mediates an embryonic lethality. Representative images of both WT and Sox10-Cre;LSL-ALK-F1174L embryo phenotypes for stages from E9.5 to E12.5. Embryos were genotyped by PCR as described in the Material and Method section. Endogenous ALK mRNA Is Detectable in Sympathetic Ganglia Starting at Stage E10.5 ALK is widely expressed in the developing nervous system. A previous report described the detection of ALK mRNA and protein in muri...
Source: Frontiers in Oncology - Category: Cancer & Oncology Source Type: research

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Publication date: Available online 9 December 2019Source: Gynecologic Oncology ReportsAuthor(s): Kumiko Seki, Hiroshi Ishikawa, Rei Hashimoto, Akira Mitsuhashi, Jun-ichiro Ikeda, Makio Shozu
Source: Gynecologic Oncology Reports - Category: OBGYN Source Type: research
Source: Critical Reviews in Food Science and Nutrition - Category: Nutrition Authors: Source Type: research
Background: The United States has the highest number of oocyte donation cycles, which account for an estimated one-quarter of all worldwide oocyte donation cycles. Although there has been a steady rise in oocyte donation treatment, understanding the kinship views of those intimately involved is lacking. These include women oocyte donors and parents who received donor oocytes to establish a pregnancy. Purpose: To explore the views and perspectives about genetic relationships and lineages among women who were oocyte donors and parents who received donated oocytes 10 to 12 years after donors and parents underwent oocyte ...
Source: MCN: The American Journal of Maternal Child Nursing - Category: Nursing Tags: Feature Source Type: research
Background: Birth fathers are the least known of the adoption triad (adoptee, birth family, adoptive family). There is a gap in evidence about birth father involvement in open adoptions. Purpose: The purpose of the study was to explore birth fathers' experiences of open adoption and contact patterns with birth children. Methods: Ten birth fathers of open adoptions were recruited from two agencies. Birth fathers participated in recorded telephone interviews using naturalistic inquiry. Verbatim transcripts were analyzed for qualitative content themes. Results: At conception and birth, birth fathers were not in a s...
Source: MCN: The American Journal of Maternal Child Nursing - Category: Nursing Tags: Feature Source Type: research
Background: African American women are more likely to experience preterm birth compared with White women. Social factors such as neighborhood disorder and experiences of racial discrimination, which disproportionately affect African American women, may partially explain these disparities. Purpose: The purpose of this study was to examine pregnant African American women's perceptions of neighborhood disorder, racial discrimination, and psychological distress and whether these concepts were viewed as influences on birth outcomes. Study Design and Methods: Using a mixed-methods approach, seven pregnant African America...
Source: MCN: The American Journal of Maternal Child Nursing - Category: Nursing Tags: Feature Source Type: research
[Daily Maverick] A doctor at 2 Military Hospital in Wynberg, Cape Town has pleaded not guilty to two charges at a Health Professions Council of SA disciplinary hearing. Jacques De Vos is accused of trying to dissuade a patient from terminating her pregnancy in December 2016 and of failing to respect the patient's autonomy.
Source: AllAfrica News: Pregnancy and Childbirth - Category: OBGYN Source Type: news
No abstract available
Source: Diseases of the Colon and Rectum - Category: Gastroenterology Tags: ASCRS Committee Report: Public Relations Source Type: research
BACKGROUND: In 2016, the American Society of Colon and Rectal Surgeons published a rectal cancer surgery checklist composed of the essential elements of preoperative, intraoperative, and postoperative care for patients undergoing rectal cancer surgery. OBJECTIVE: The purpose of this study was to assess whether compliance with preoperative checklist elements was associated with improved pathologic and 30-day postoperative outcomes after rectal cancer surgery. DESIGN: This was a retrospective cohort study. SETTINGS: The study involved North American hospitals contributing to the American College of Surgeons Nation...
Source: Diseases of the Colon and Rectum - Category: Gastroenterology Tags: Original Contributions: Colorectal Cancer Source Type: research
CONCLUSIONS: The residual disease rate in patients treated surgically was higher than previously reported (43.2%). Incidence of recurrence in patients treated conservatively was low (4.4%). Areas of improvements have been identified in adherence to endoscopic follow-up, histopathological reporting, and potential overuse of radiological surveillance. See Video Abstract at http://links.lww.com/DCR/B47. MANEJO ACTUAL DE PÓLIPOS COLORRECTALES MALIGNOS A TRAVÉS DE UNA RED REGIONAL DE CÁNCER DEL REINO UNIDO ANTECEDENTES: La incidencia de pólipos colorrectales malignos ha aumentado secundariament...
Source: Diseases of the Colon and Rectum - Category: Gastroenterology Tags: Original Contributions: Colorectal Cancer Source Type: research
CONCLUSIONS: Resections that were approached laparoscopically were more likely to achieve oncologic success. Minimally invasive approaches did not lengthen operative times and provided benefits of reduced surgical site complications and decreased postoperative length of stay. Further studies are needed to clarify clinical outcomes and factors that influence the choice of approach. See Video Abstract at http://links.lww.com/DCR/B70. RESULTADOS ONCOLÓGICOS Y PERIOPERATORIOS DE LOS ABORDAJES LAPAROSCÓPICOS, ABIERTOS Y ROBÓTICOS PARA LA RESECCIÓN DEL CÁNCER RECTAL: UN ESTUDIO DE COHORTE MU...
Source: Diseases of the Colon and Rectum - Category: Gastroenterology Tags: Original Contributions: Colorectal Cancer Source Type: research
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