Group 2 Innate Lymphoid Cells Are Redundant in Experimental Renal Ischemia-Reperfusion Injury

In this study, we sought to further characterize ILC2s in the kidney, their location within this organ and determine their functional role in IRI using a loss-of-function approach. Here, we found that kidney ILC2s constitutively express IL-5 and are primarily located in close proximity to the renal vasculature, within the adventitia. Additionally, we demonstrate that a reduction, deficiency or depletion of ILC2s had minimal impact on the severity of IRI. Whilst activation of ILC2s and the associated amplification of local type 2 immunity has been previously shown to reduce the deleterious consequences of AKI, our results reveal that comparable injury occurs in the absence of ILC2s, suggesting that ILC2s may be redundant in IRI when other compensatory immune cells such as T cells are present. Materials and Methods Mice Eight to twelve week-old male wild-type (WT; C57BL/6JAusb), vehicle (saline-treated Icosdtr/+Cd4cre/+), ILC2-reduced (Rorafl/+Il7rcre/+), ILC2-deficient (Rorafl/flIl7rcre/+), ILC2-depleted (Diptheria toxoid-treated [DTx] Icosdtr/+ Cd4cre/+), and IL-5/IL-13 dual reporter mice (Il5venus/+Il13td−tomato/+) mice were obtained from Australian Bioresources (Moss Vale, Australia). Il5td−tomatoCre; Rosa26-CAG-RFP reporter-tracker mice were obtained from Jackson Labs (ref# 030926 and 007914). All mice that underwent surgery were housed under specific pathogen free, physical containment 2 conditions, in individ...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research