Group 2 Innate Lymphoid Cells Are Redundant in Experimental Renal Ischemia-Reperfusion Injury

In this study, we sought to further characterize ILC2s in the kidney, their location within this organ and determine their functional role in IRI using a loss-of-function approach. Here, we found that kidney ILC2s constitutively express IL-5 and are primarily located in close proximity to the renal vasculature, within the adventitia. Additionally, we demonstrate that a reduction, deficiency or depletion of ILC2s had minimal impact on the severity of IRI. Whilst activation of ILC2s and the associated amplification of local type 2 immunity has been previously shown to reduce the deleterious consequences of AKI, our results reveal that comparable injury occurs in the absence of ILC2s, suggesting that ILC2s may be redundant in IRI when other compensatory immune cells such as T cells are present. Materials and Methods Mice Eight to twelve week-old male wild-type (WT; C57BL/6JAusb), vehicle (saline-treated Icosdtr/+Cd4cre/+), ILC2-reduced (Rorafl/+Il7rcre/+), ILC2-deficient (Rorafl/flIl7rcre/+), ILC2-depleted (Diptheria toxoid-treated [DTx] Icosdtr/+ Cd4cre/+), and IL-5/IL-13 dual reporter mice (Il5venus/+Il13td−tomato/+) mice were obtained from Australian Bioresources (Moss Vale, Australia). Il5td−tomatoCre; Rosa26-CAG-RFP reporter-tracker mice were obtained from Jackson Labs (ref# 030926 and 007914). All mice that underwent surgery were housed under specific pathogen free, physical containment 2 conditions, in individually ventilated cages. Mice were exposed to nor...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research

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Publication date: Available online 21 October 2019Source: Best Practice &Research Clinical AnaesthesiologyAuthor(s): Girish P. Joshi, Francis Bonnet
Source: Best Practice and Research Clinical Anaesthesiology - Category: Anesthesiology Source Type: research
ConclusionsThis case series demonstrates that the use of a high frequency jet ventilator for cardiac MRI was feasible, safe, providing good quality cardiac imaging and avoiding anesthesia personnel to be inside the hazardous environment of MRI room. Future studies are needed to confirm its safety and efficiency in pediatric patients.ResumoJustificativa e objetivosA ressonância magnética (RM) cardíaca é uma técnica utilizada na avaliação de crianças com cardiopatias congênitas. A anestesia geral garante imobilidade, especialmente em pacientes não cooperador...
Source: Brazilian Journal of Anesthesiology - Category: Anesthesiology Source Type: research
This article focuses on the functional features of positive-pressure ventilators, the modes of invasive and non-invasive mechanical ventilation, and the main ventilator settings. It also highlights the potential complications of mechanical ventilation, the basic principles of weaning, and the pathophysiological basis of patient-ventilator dyssynchrony.
Source: Anaesthesia and intensive care medicine - Category: Anesthesiology Source Type: research
Publication date: Available online 22 October 2019Source: Autoimmunity ReviewsAuthor(s): Daniel Blockmans, Raashid Luqmani, Lucia Spaggiari, Carlo SalvaraniAbstractWith advances in our understanding of the pathogenesis of large vessel vasculitides, we recognise the persistence of inflammation in large vessels, sometimes despite therapy to control clinical symptoms. Achieving an early diagnosis and establishing the extent of disease are important steps in improving our management of these diseases. Imaging is playing an increasing role in the assessment of these patients from diagnosis to prognosis. We review the current an...
Source: Autoimmunity Reviews - Category: Allergy & Immunology Source Type: research
Publication date: Available online 21 October 2019Source: Autoimmunity ReviewsAuthor(s): Piercarlo Sarzi-Puttini, Jacob Ablin, Adva Trabelsi, Mary-Ann Fitzcharles, Daniela Marotto, Winfried HäuserAbstractMedical cannabis is being increasingly used in the treatment of rheumatic diseases because, despite the paucity of evidence regarding its safety and efficacy, a growing number of countries are legalising its use for medical purposes in response to social pressure. Cannabinoids may be useful in the management of rheumatic disorders for two broad reasons: their anti-inflammatory and immunomodulatory activity, and their ...
Source: Autoimmunity Reviews - Category: Allergy & Immunology Source Type: research
Low English proficiency puts patients with chronic conditions such as heart failure and COPD at much higher risk for returning to the hospital, new data show.Medscape Medical News
Source: Medscape Allergy Headlines - Category: Allergy & Immunology Tags: Hospital Medicine News Source Type: news
Conclusion: The relative proportion of IPUD is increasing in our population in contrast to the drop of H. pylori-PUD. Treatment with high-dose PPI, and for a long duration, heals IPUD and protects from recurrence. PMID: 31630582 [PubMed - as supplied by publisher]
Source: Scandinavian Journal of Gastroenterology - Category: Gastroenterology Tags: Scand J Gastroenterol Source Type: research
Conclusions: Patients with clinical signs of inflammation and acute PVT have a low risk of malignancy in contrast to patients without signs of inflammation and acute PVT, which have a high risk of oncologic or hemato-oncologic disease. Patients with negative hemato-oncologic screening should be carefully observed over time because we expect they are at higher risk for the development of hemato-oncologic disease, independent from the presence and number of procoagulation risk factors. PMID: 31630579 [PubMed - as supplied by publisher]
Source: Scandinavian Journal of Gastroenterology - Category: Gastroenterology Tags: Scand J Gastroenterol Source Type: research
Conclusions: The results indicate that a possible mechanism behind an anastomotic leakage is an impaired circulation and thus insufficient saturation to the small intestine peroperatively. This develops into an inflammation both intraperitoneally and intravenously, which, if not reversed, spread within the gastrointestinal tract .The colorectal anastomosis is the most vulnerable part of the gastrointestinal tract postoperatively and hypoxia and inflammation may occur there, and an anastomosis leakage will be the consequence. PMID: 31630578 [PubMed - as supplied by publisher]
Source: Scandinavian Journal of Gastroenterology - Category: Gastroenterology Tags: Scand J Gastroenterol Source Type: research
Publication date: Available online 21 October 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Wesley L. Fotoran, Thomas Müntefering, Nicole Kleiber, Beatriz N.M. Miranda, Eva Liebau, Darrell J. Irvine, Gerhard WunderlichAbstractLipid particles for drug delivery can be modified to create multilayer vesicles with higher stability and improved cargo interaction. Here, we used lipids capable of forming hydrogen bonds instead of covalent bonds and designed stable vesicles-inside-vesicles with a high capacity of entrapping antimalarial drugs such as chloroquine (hydrophilic) and Artemisinin (lipophi...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - Category: Nanotechnology Source Type: research
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