Endothelial cell-derived small extracellular vesicles suppress cutaneous wound healing through regulating fibroblasts autophagy

Diabetic foot ulcer is a life-threatening clinical problem in diabetic patients. Endothelial cell-derived small extracellular vesicles (sEVs) are important mediators of intercellular communication in the pathogenesis of several diseases. However, the exact mechanisms of wound healing mediated by endothelial cell-derived sEVs remain unclear. sEVs were isolated from human umbilical vein endothelial cells (HUVECs) pretreated with or without advanced glycation end products (AGEs). The roles of HUVECs-derived sEVs on the biological characteristics of skin fibroblasts were investigated both in vitro and in vivo. We demonstrate that sEVs derived from AGEs-pretreated HUVECs (AGEs-sEVs) could inhibit collagen synthesis by activating autophagy of human skin fibroblasts. Additionally, treatment with AGEs-sEVs could delay the wound healing process in SD rats. Further analysis indicated that miR-106b-5p was upregulated in AGEs-sEVs and importantly, in exudate derived sEVs from patients with diabetic foot ulcer. Consequently, sEVs-mediated uptake of miR-106b-5p in recipient fibroblasts reduces expression of ERK1/2, resulting in fibroblasts autophagy activation and subsequent collagen degradation. Collectively, our data demonstrate that miR-106b-5p could be enriched in AGE-sEVs, then decreases collagen synthesis and delays cutaneous wound healing by triggering fibroblasts autophagy through reducing ERK1/2 expres...
Source: Clinical Science - Category: Biomedical Science Authors: Tags: PublishAheadOfPrint Source Type: research

Related Links:

Abstract Diabetic foot ulcer (DFU) is a complication experienced by diabetic patients and does not heal well in an altered wound environment. Although diverse microbes in DFU were detected, little is known about their influences on diabetic foot wound (DFW) and the association with the skin microbiota in normal tissue from the same patients according to clinical features. We aimed to analyze the microbiota in normal skin and DFW tissue from the same subject and predict their roles based on clinical features. We analyzed the microbiota in normal skin and DFW tissue from the same subject and compared the associated ...
Source: Biomed Res - Category: Research Authors: Tags: Biomed Res Int Source Type: research
Condition:   Diabetic Foot Ulcer Intervention:   Device: Negative Pressure Wound Therapy Sponsor:   Assiut University Not yet recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
This study aimed to determine the seizure frequency and assess the clinical benefit of a 5-minute air-break (5´-AIRBK). METHODS: Twenty-year (1999-2018) retrospective analysis of all consecutive treatments with HBOT. Medical records were reviewed to determine patient demographics, comorbidities, HBOT indications, and seizure characteristics and timing. Seizure frequency was compared before and after incorporating a 5´-AIRBK in the treatment protocol. Chi-square testing was performed using SPSS (version 24.0); P
Source: Diving and Hyperbaric Medicine - Category: Sports Medicine Tags: Diving Hyperb Med Source Type: research
Acta Pharmacologica Sinica, Published online: 18 September 2019; doi:10.1038/s41401-019-0288-7Silicone elastomer gel impregnated with 20(S)-protopanaxadiol-loaded nanostructured lipid carriers for ordered diabetic ulcer recovery
Source: Acta Pharmacologica Sinica - Category: Drugs & Pharmacology Authors: Source Type: research
Condition:   Diabetic Foot Interventions:   Drug: PRP gel;   Drug: Saline dressing Sponsor:   Mansoura University Completed
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Because collagen is fundamental to wound healing and skin formation, collagen-containing dressing materials might be beneficial in treating diabetic foot ulcers (DFU), but supporting evidence is needed. Here, we examined the effectiveness and safety of collagen dressing material in DFU treatment.
Source: Diabetes Research and Clinical Practice - Category: Endocrinology Authors: Source Type: research
This study aimed to clinically assess the efficiency of Quercus infectoria solution for the treatment of chronic diabetic ulcers in patients. Quercus infectoria solution was pharmaceutically formulated. A multicenter randomized controlled trial was performed in diabetic patients with ulcers (Wagner Ulcer Classification grade 1-3), who received standard wound treatment (normal saline solution) plus Quercus infectoria solution (study group; n = 26) or the standard solution alone (control group; n = 25) for three months. The primary outcome measure was complete wound healing. Percentage reduction i...
Source: Journal of Herbal Medicine - Category: Complementary Medicine Source Type: research
In conclusion, miR-23c may function as a new regulator to inhibit angiogenesis by targeting SDF-1α.Graphical abstract
Source: Microvascular Research - Category: Biochemistry Source Type: research
Journal of Wound Care,Volume 28, Issue 9, Page 601-607, September 2019.
Source: Journal of Wound Care - Category: Nursing Authors: Source Type: research
Condition:   Diabetic Foot Interventions:   Device: Sealed therapeutic shoe;   Device: Total contact cast Sponsors:   Region Örebro County;   Stiftelsen Promobila;   FORTE Recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
More News: Biomedical Science | Diabetes | Diabetic Ulcers | Endocrinology | Science | Skin