Endothelial cell-derived small extracellular vesicles suppress cutaneous wound healing through regulating fibroblasts autophagy

Diabetic foot ulcer is a life-threatening clinical problem in diabetic patients. Endothelial cell-derived small extracellular vesicles (sEVs) are important mediators of intercellular communication in the pathogenesis of several diseases. However, the exact mechanisms of wound healing mediated by endothelial cell-derived sEVs remain unclear. sEVs were isolated from human umbilical vein endothelial cells (HUVECs) pretreated with or without advanced glycation end products (AGEs). The roles of HUVECs-derived sEVs on the biological characteristics of skin fibroblasts were investigated both in vitro and in vivo. We demonstrate that sEVs derived from AGEs-pretreated HUVECs (AGEs-sEVs) could inhibit collagen synthesis by activating autophagy of human skin fibroblasts. Additionally, treatment with AGEs-sEVs could delay the wound healing process in SD rats. Further analysis indicated that miR-106b-5p was upregulated in AGEs-sEVs and importantly, in exudate derived sEVs from patients with diabetic foot ulcer. Consequently, sEVs-mediated uptake of miR-106b-5p in recipient fibroblasts reduces expression of ERK1/2, resulting in fibroblasts autophagy activation and subsequent collagen degradation. Collectively, our data demonstrate that miR-106b-5p could be enriched in AGE-sEVs, then decreases collagen synthesis and delays cutaneous wound healing by triggering fibroblasts autophagy through reducing ERK1/2 expres...
Source: Clinical Science - Category: Biomedical Science Authors: Tags: PublishAheadOfPrint Source Type: research

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Authors: Liu LN, Wang L, Jia SJ, Wang P Abstract BACKGROUND The aims of this study were to summarize the clinical characteristics and risk factors for bezoars and to analyze the effectiveness and safety of the endoscopic treatment of bezoars. MATERIAL AND METHODS From January 2015 to February 2020, 75 of the 23 950 patients who underwent gastroscopic examination in our medical center were diagnosed with bezoars. Clinical and treatment information for these patients was collected retrospectively and analyzed. RESULTS The detection rate of bezoars was 0.31%. Risk factors included the time of year (autumn and winter s...
Source: Medical Science Monitor - Category: Research Tags: Med Sci Monit Source Type: research
Honey with tea, toast, apples and oatmeal – that all sounds normal and delicious. Honey on wounds, though? It may sound a little out of the ordinary, but honey has actually helped wounds heal for thousands of years. While there are plenty of advanced healing tools available today, honey's antibacterial properties and unique pH balance still make it a helpful treatment for certain wounds. Here's what to know about using honey for healing wounds: Honey has powerful healing properties. The type of honey Wound-care professionals typically use medical-grade honey for healing chronic wounds, although Manuka ...
Source: Advanced Tissue - Category: Dermatology Authors: Tags: Wound healing Wound care products Source Type: news
Conclusions: To rationalize use and distribution of medical resources, we suggest proper regular care and health education to those with mental disabilities and multiple chronic conditions, which may not only improve the situation of highly frequent hospital visits but also effectively control incidence of acute conditions and reduce their ED visits. In addition, improving the continuity of care is vital, and the best way to do this is to promote family medicine as the primary form of healthcare. PMID: 33017273 [PubMed - as supplied by publisher]
Source: Current Medical Research and Opinion - Category: Research Tags: Curr Med Res Opin Source Type: research
Publication date: Available online 5 October 2020Source: Canadian Journal of DiabetesAuthor(s): Jérôme Patry, André Tourigny, Marie-Philippe Mercier, Clermont E. Dionne
Source: Canadian Journal of Diabetes - Category: Endocrinology Source Type: research
The prospect of an increase in ageing population and incidence of diabetes potentially increases the number of amputees in the future1. Hip fractures in lower limb amputees have been reported sporadically in literature2. Amputees suffer from accelerated bone density loss regardless of their age and are at increased risk of osteoporosis and fragility fractures of the hip2,3. This also increases the risk of other co-morbidities such as pressure ulcers and infection if the treatment is delayed4. Hip fractures amongst lower limb amputees though uncommon, pose a management challenge with regards to the surgical approach and tec...
Source: Injury - Category: Orthopaedics Authors: Source Type: research
Journal of Wound Care,Volume 29, Issue Sup10a, Page S15-S16, October 2020.
Source: Journal of Wound Care - Category: Nursing Authors: Source Type: research
Journal of Wound Care,Volume 29, Issue Sup10a, Page S17-S18, October 2020.
Source: Journal of Wound Care - Category: Nursing Authors: Source Type: research
Abstract AIMS: To investigate the risk of corneal ulcer in patients with atopic keratoconjunctivitis (AKC). METHODS: The nationwide, population-based, retrospective, matched cohort study included 171 019 newly diagnosed patients with AKC who were identified by the International Classification of Diseases, Ninth Revision, Clinical Modification (ICD-9-CM), code 372.05, and selected from the Taiwan National Health Insurance Research Database. The age-, sex- and potential comorbidities-matched control group included 171 019 patients with non-AKC selected from the Taiwan Longitudinal Health Insurance Dat...
Source: The British Journal of Ophthalmology - Category: Opthalmology Authors: Tags: Br J Ophthalmol Source Type: research
CONCLUSIONS: The comprehensive CAP study landscape with structured case report summaries and randomized case-control studies allows the conclusion that CAP is safe, effective, and easy to handle for wound treatment. The utilization of CAP in addition to standard wound treatments is starting to enter routine clinical practice. PMID: 32997219 [PubMed - as supplied by publisher]
Source: Der Hautarzt: Zeitschrift fur Dermatologie, Venerologie, und verwandte Gebiete - Category: Dermatology Tags: Hautarzt Source Type: research
Condition:   Diabetic Foot Ulcer Interventions:   Biological: ALLO-ASC-DFU;   Procedure: Vehicle Sheet Sponsor:   Anterogen Co., Ltd. Recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
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