Inhibition of miR-140-3p or miR-155-5p by antagomir treatment sensitize chordoma cells to chemotherapy drug treatment by increasing PTEN expression.
Inhibition of miR-140-3p or miR-155-5p by antagomir treatment sensitize chordoma cells to chemotherapy drug treatment by increasing PTEN expression.
Eur J Pharmacol. 2019 Apr 10;:
Authors: Zhao K, Li X, Chen X, Zhu Q, Yin F, Ruan Q, Xia J, Niu Z
Abstract
Previous researches suggested microRNA-140-3p (miR-140-3p) and miR-155-5p as cancer promotor in chordoma. We aimed to investigate the mechanisms of these two miRNAs in chordoma cells. Patient-derived chordoma cell lines were established in vitro. Expressions of miR-140-3p or miR-155-5p were measured by quantitative real-time polymerase chain reaction and their functions were inhibited by antagomir treatment. Malignancy of chordoma cells was assessed by cell viability, proliferation, apoptosis, colony formation and transwell invasion assays as well as western blot evaluating epithelial-to-mesenchymal transition. Sensitivity of chordoma cells was assessed by cell viability and apoptosis assays. Protein level of phosphatase and tensin homolog (PTEN) and phosphoinositide 3-kinase (PI3K)- protein kinase B (Akt)-mammalian target of rapamycin (mTOR) pathway were assessed by western blot. Interaction of miR-140-3p and miR-155-5p with the 3' untranslated region of PTEN mRNA was verified by luciferase reporter assay. BpV was used to inhibit PTEN activity. The expressions of miR-140-3p and miR-155-5p were enhanced in chordoma cells and inhibited by treatment of antagomirs. Inhibition of miR-140...
Source: European Journal of Pharmacology - Category: Drugs & Pharmacology Authors: Zhao K, Li X, Chen X, Zhu Q, Yin F, Ruan Q, Xia J, Niu Z Tags: Eur J Pharmacol Source Type: research
More News: Cancer | Cancer & Oncology | Chemotherapy | Chordoma | Drugs & Pharmacology | Epithelial Cancer | Science