Biofilm formation and synthesis of antimicrobial compounds by the biocontrol agent Bacillus velezensis QST713 in Agaricus bisporus compost micromodel.

We report an over-expression of several genes of the biocontrol agent involved in biofilm formation in the compost compared to expression during growth in broth compost extract, either in the exponential growth phase (epsC, blsA and tapA genes) or in the stationary growth phase (tapA gene), while a gene encoding a flagellar protein (hag) was under-expressed. We also report an over-expression of Bacillus velezensis QST713 genes related to surfactin (srfAA) and fengycin (fenA) production in the presence of the fungal pathogen in the compost.ImportanceBiocontrol agents are increasingly used to replace chemical pesticides to prevent crop diseases. In the button mushroom field in France, the use of Bacillus velezensis QST713 as a biocontrol agent against the green mold Trichoderma aggressivum, has been shown to be efficient. However, the biocontrol mechanisms effective in the pathosystem Agaricus bisporus/Trichoderma aggressivum/Bacillus velezensis QST713 are still unknown. Our paper focuses on the exploration of the bioprotection mechanisms of the biocontrol agent Bacillus velezensis QST713 during the culture of the button mushroom (Agaricus bisporus) in a micromodel culture system to study the specific response of strain QST713 in the presence of T. aggressivum and/or A. bisporus. PMID: 30979839 [PubMed - as supplied by publisher]
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research