Conservation of chicken male germ line by orthotopic transplantation of primordial germ cells from genetically distant donors †.

Conservation of chicken male germ line by orthotopic transplantation of primordial germ cells from genetically distant donors†. Biol Reprod. 2019 Apr 13;: Authors: Mucksová J, Reinišová M, Kalina J, Lejčková B, Hejnar J, Trefil P Abstract Successful derivation and cultivation of primordial germ cells (PGC) opened the way to efficient transgenesis and genome editing in the chicken. Furthermore, implantation of male PGCs from non-chicken galliform species into the chicken embryos resulted in cross-species germ line chimeras and viable offspring. We have recently improved the PGC technology by demonstrating that chicken male PGCs transplanted into the testes of adult cockerel recipients mature into functional sperms. However, the availability of this orthotopic transplantation for cross-species transfer remains to be explored. Here we tested the capacity of genetically distant male PGCs to mature in the microenvironment of adult testes. We derived PGCs from the Chinese black-bone Silkie and transplanted them into infertile White Leghorn cockerels. Within 15-18 weeks after transplantation, we observed restoration of spermatogenesis in recipient cockerels and production of healthy progeny derived from the transplanted PGCs. Our findings also indicate the possibility of cross-species orthotopic transplantation of PGCs. Thus, our results might contribute to the preservation of endangered avian species and maintaining the gene...
Source: Biology of Reproduction - Category: Reproduction Medicine Authors: Tags: Biol Reprod Source Type: research

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CONCLUSIONS In a summary, by inhibiting the STAT3 activation, esculetin shows potential anticancer effects against the laryngeal cancer. PMID: 31630150 [PubMed - in process]
Source: Medical Science Monitor - Category: Research Tags: Med Sci Monit Source Type: research
Changes in indices of mineral metabolism, bone protein expression, and bone turnover were assessed between pre – and post–renal transplant bone biopsies obtained 12 months apart. Circulating sclerostin and fibroblast growth factor 23 (FGF-23) levels decreased, and a low bone turnover state was highly prevalent on follow-up. In contrast, bone sclerostin expression increased, whereas FGF-23 bone expression was unchanged/decreased. These findings underscore the limitations of circulating biomarkers and the critical role of bone biopsy to understand osteocyte biology in chronic kidney disease–mineral bone disorder.
Source: Kidney International - Category: Urology & Nephrology Authors: Tags: Commentary Source Type: research
A 63-year-old man was admitted 2 months after a deceased-donor kidney transplantation with an acute rise of his creatinine from a baseline of 1.9 mg/dl to 3.7 mg/dl. The patient had received basiliximab induction, followed by maintenance with belatacept, mycophenolic acid, and steroids. Physical examination and an ultrasound of the kidney transplant were unremarkable. Kidney biopsy revealed Banff acute cellular rejection grade IIB. Belatacept was replaced with tacrolimus, and steroid pulse and rabbit antithymocyte globulin were administered.
Source: Kidney International - Category: Urology & Nephrology Authors: Tags: Nephrology Image Source Type: research
A 36-year-old female recipient of simultaneous pancreas-kidney transplantation 2 months previously was admitted to the emergency room because of a fever of unknown origin. Physical examination was unremarkable. Klebsiella pneumoniae was isolated in urine culture and treated with ceftriaxone according to antibiogram. High-grade fever persisted despite the increase in antibiotic spectrum with meropenem. Blood cultures were negative (including mycobacteria), as were quantiferon, cytomegalovirus, BK virus, Epstein –Barr virus polymerase chain reaction, and Toxoplasma gondii serologies.
Source: Kidney International - Category: Urology & Nephrology Authors: Tags: Nephrology Image Source Type: research
Conclusions: The 7th edition of the TNM classification for esophageal carcinoma is poorly recognized and understood in central and southern China, which might contribute to the relatively low rates of appropriate perioperative procedures applied for esophageal cancer patients.
Source: Frontiers in Oncology - Category: Cancer & Oncology Source Type: research
The follicle-associated epithelium (FAE) is a specialized structure that samples luminal antigens and transports them into mucosa-associated lymphoid tissues (MALT). In mammals, transcytosis of antigens across the gut epithelium is performed by a subset of FAE cells known as M cells. Here we show that colony-stimulating factor 1 receptor (CSF1R) is expressed by a subset of cells in the avian bursa of Fabricius FAE. Expression was initially detected using a CSF1R-reporter transgene that also label subsets of bursal macrophages. Immunohistochemical detection using a specific monoclonal antibody confirmed abundant expression ...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
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Source: Frontiers in Psychiatry - Category: Psychiatry Source Type: research
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Source: Frontiers in Endocrinology - Category: Endocrinology Source Type: research
(Natural News) The World Health Organization, a criminal cartel front for the vaccine industry, has just declared war on the so-called “anti-vax” movement. The W.H.O. is the same group that spiked vaccines given to women in Africa with abortion / infertility drugs. The W.H.O. is also the same fraudulent group that falsely declared H5N1 bird...
Source: NaturalNews.com - Category: Consumer Health News Source Type: news
Abstract All mammalian eggs are surrounded by a highly specialized extracellular matrix (ECM), called the zona pellucida (ZP), that functions before, during, and after fertilization. Unlike somatic cell ECM the mouse ZP is composed of three different proteins, ZP1-3, that are synthesized and secreted by growing oocytes and assembled into long interconnected fibrils. ECM or vitelline envelope (VE) that surrounds fish, reptilian, amphibian, and avian eggs also consists of a limited number of proteins all closely related to ZP1-3. Messenger RNAs encoding ZP1-3 are expressed only by growing oocytes at very high levels...
Source: Current Topics in Developmental Biology - Category: Biology Authors: Tags: Curr Top Dev Biol Source Type: research
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