Temporal analysis of distribution pattern of islet cells and antioxidant enzymes for diabetes onset in postnatal critical development window in rats

Publication date: 1 June 2019Source: Life Sciences, Volume 226Author(s): Franciane Quintanilha Gallego, Carolina Abreu Miranda, Yuri Karen Sinzato, Isabela Lovizutto Iessi, Bruna Dallaqua, Rogelio Hernandez Pando, Noeme Sousa Rocha, Gustavo Tadeu Volpato, Débora Cristina DamascenoAbstractAimAt performing a temporal analysis of the distribution pattern of islet endocrine cells and antioxidant enzymes in diabetic rats during the post-natal critical development window.Main methodsThe newborns received streptozotocin (STZ) at birth for diabetes induction, and control females received the vehicle. The animals were euthanized at different lifetimes: D5, D10, D15, and D30. Morphological analysis of pancreas and biochemical assays was performed.Key findingsThe STZ-induced rats presented irregular shape of islet on D5 and there was an attempt to restore of this shape in other life moment studied. There was an increase progressive in islet area, however they maintained smaller than those of control rats, with lower labeling intensity for insulin, higher for glucagon and somatostatin, lower for SOD-1 was lower in the islets of the STZ-induced animals at all times studied and for GSH-Px in D10 and D30.SignificanceAlthough STZ-induced diabetic rats presented compensatory mechanisms to restore the mass of endocrine cells, this was not sufficient since these rats developed the diabetic state. This was confirmed by the oral glucose tolerance test from D30. In addition, the delta (δ)-cells ...
Source: Life Sciences - Category: Biology Source Type: research