Comprehensive Kinetic Survey of Intestinal, Extra-Intestinal and Systemic Sequelae of Murine Ileitis Following Peroral Low-Dose Toxoplasma gondii Infection

Comprehensive Kinetic Survey of Intestinal, Extra-Intestinal and Systemic Sequelae of Murine Ileitis Following Peroral Low-Dose Toxoplasma gondii Infection Markus M. Heimesaat1*, Ildiko R. Dunay2 and Stefan Bereswill1 1Institute of Microbiology, Infectious Diseases and Immunology, Charité - University Medicine Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany2Medical Faculty, Institute of Inflammation and Neurodegeneration, University Hospital Magdeburg, Magdeburg, Germany We have recently shown that following peroral low-dose Toxoplasma gondii infection susceptible mice develop subacute ileitis within 10 days. Data regarding long-term intestinal and extra-intestinal sequelae of infection are scarce, however. We therefore challenged conventional C57BL/6 mice with one cyst of T. gondii ME49 strain by gavage and performed a comprehensive immunopathological survey 10, 36, and 57 days later. As early as 10 days post-infection, mice were suffering from subacute ileitis as indicated by mild-to-moderate histopathological changes of the ileal mucosa. Furthermore, numbers of apoptotic and proliferating/regenerating epithelial cells as well as of T and B lymphocytes in the mucosa and lamina propria of the ileum were highest at day 10 post-infection, but declined thereafter, and were accompanied by enhanced pro-inflammatory mediator secretion in ileum, colon and mesenteric lymph n...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research

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Publication date: Available online 14 December 2019Source: Journal of Photochemistry and Photobiology B: BiologyAuthor(s): Jiahua Shao, Zheru Ding, Lexiang Li, Yi Chen, Jun Zhu, Qirong QianAbstractArticular cartilage regeneration is a challenging process due to its inadequate ability of self-recovering biological mechanisms. The progresses of cartilage tissue engineering is supported to overwhelmed the repairing difficulties and degenerative diseases. The main goal of the present study is to design biomaterials with suitable physico-chemical, mechanical and biological properties for the carrier of growth factor and improvi...
Source: Journal of Photochemistry and Photobiology B: Biology - Category: Speech-Language Pathology Source Type: research
ConclusionIn orthopaedics, bone defects create a high impact on the quality of life of the patient. It leads to a higher demand for bone substitutes for replacement of bone defect. Bone tissue engineering can help to replace a critical defect bone. 3D printing is a useful technology for the fabrication of scaffolds critical in bone tissue engineering. There are different binders which can create bone scaffolds with requisite mechanical strength. These binders are used to create excellent osteoconductive, bioactive scaffolds. Computed tomography (CT) and Magnetic resonance imaging (MRI) help to provide images of specific de...
Source: Journal of Clinical Orthopaedics and Trauma - Category: Orthopaedics Source Type: research
A patent dispute means that the marketing of a biosimilar for patients with inflammatory bowel disease will be delayed, despite approval by the US Food and Drug Administration, investigators report.Medscape Medical News
Source: Medscape Medical News Headlines - Category: Consumer Health News Tags: Gastroenterology News Source Type: news
Publication date: January 2020Source: Colloid and Interface Science Communications, Volume 34Author(s): Tatiane Melo Pereira, Vera Lucia Perussi Polez, Marcelo Henrique Sousa, Luciano Paulino SilvaAbstractGreen synthesis of silver nanoparticles (AgNPs) is already widely used, however, it presents challenges. This work describes a way to modulate the physicochemical characteristics of AgNPs using green synthesis and aqueous extract from different parts of Handroanthus heptaphyllus. The progress of the synthesis reaction, the physicochemical characteristics, and the biological activity against Escherichia coli and Staphyloco...
Source: Colloids and Interface Science Communications - Category: Nanotechnology Source Type: research
In this study, SS attached to plant bodies were subjected to acid and heat treatments, followed by grinding, to obtain 10–40-nm siliceous sponge spicules (SSS). SSS and polylactic acid (PLA) were then combined to create 50–450-nm PLA/SSS composite nanofibers. The morphology and bioactivity of the electrospun PLA/SSS nanofibers were examined; the tensile, thermal, and water-resistant properties of the fibers were also evaluated. Our results showed a dramatic enhancement in the thermal and tensile properties of PLA with increasing SSS content; specifically, a 3 wt% increase in SSS content resulted in a 47&nb...
Source: Materials Science and Engineering: C - Category: Materials Science Source Type: research
Abstract Given that the C580Y polymorphism in the Plasmodium falciparum propeller domain of the kelch 13 gene (pfk13) was documented in Guyana, monitoring for mutations associated with antimalarial resistance was undertaken in neighboring Roraima state in Brazil. Polymorphisms in the pfmdr1 and pfk13 genes were investigated in 275 P. falciparum samples. No pfk13 mutations were observed. Triple mutants 184F, 1042D, and 1246Y were observed in 100% of the samples successfully sequenced for the pfmdr1 gene, with 20.1% of these having an additional mutation at codon 1034C. Among them, 2.5% of samples harbored two copie...
Source: The American Journal of Tropical Medicine and Hygiene - Category: Tropical Medicine Authors: Tags: Am J Trop Med Hyg Source Type: research
CONCLUSIONS: AI technologies are growing their role in health care; but, up to now, their "real-life" implementation remains limited. However, in the near future, the potential of AI-driven era could change the clinical practice in Urology, improving overall patient outcomes. PMID: 31833725 [PubMed - as supplied by publisher]
Source: Minerva Urologica e Nefrologica - Category: Urology & Nephrology Tags: Minerva Urol Nefrol Source Type: research
CONCLUSIONS: About one to five patients treated with URS developed an infectious complication and E. Faecalis and E. Coli were the most frequent pathogen isolated. A positive SC is the only independent risk factor for postoperative infection: consequently, an early SC analysis could allow a prompt antibiotic therapy in all patients with positive SC even if mildly symptomatic. PMID: 31833334 [PubMed - as supplied by publisher]
Source: Minerva Urologica e Nefrologica - Category: Urology & Nephrology Tags: Minerva Urol Nefrol Source Type: research
Publication date: January 2020Source: The Annals of Thoracic Surgery, Volume 109, Issue 1Author(s): Muhammad Aftab, T. Brett Reece, Lars G. Svensson
Source: The Annals of Thoracic Surgery - Category: Cardiovascular & Thoracic Surgery Source Type: research
ConclusionsSarcopenia is prevalent in LTx patients. Neither sarcopenia nor 6MWD predicted mortality or short-term outcomes after LTx. This is in contrast to albumin levels, which were inversely associated with survival and complications. Albumin shows promise as an important predictor of mortality and short-term outcomes after LTx.
Source: The Annals of Thoracic Surgery - Category: Cardiovascular & Thoracic Surgery Source Type: research
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