The Cord Blood Insulin and Mitochondrial DNA Content Related Methylome

In conclusion, in our investigation of epigenome-wide methylation, we identified several previously described as well as novel genes and pathways on multiple levels in relation to both, cord blood insulin and mtDNA content. Possible important targets of insulin as well as mitochondrial (dys-) function during embryogenesis point in the direction of neuronal development, histone modification, CYP-metabolism, and biological aging. Ethics Statement This study was carried out in accordance with the recommendations of “Comité voor Medische Ethiek UHasselt” and “Comité Medische Ethiek ZOL” with written informed consent from all subjects. All subjects gave written informed consent in accordance with the Declaration of Helsinki. The protocol was approved by the “Comité voor Medische Ethiek UHasselt” and “Comité Medische Ethiek ZOL”. Author Contributions BR, MP, and TN designed the research hypothesis. BR, RA, and MP analyzed the data, interpreted the results, and drafted the article. JP, BJ, and NS performed the laboratory analysis of the samples with regard to cord blood insulin levels and mtDNA content. PV, AEP, TdK, AG, and ZH were responsible for the analyses of DNA methylation and the transcriptome. TN, PV, MC-H, OR, and BC provided critical revision of the manuscript with respect to the content. All authors read and approved the final manuscript. Funding The ENVIRONAGE birth cohort is suppo...
Source: Frontiers in Genetics - Category: Genetics & Stem Cells Source Type: research