Cancers, Vol. 11, Pages 525: Transient Receptor Potential Mucolipin-1 Channels in Glioblastoma: Role in Patient ’s Survival

Cancers, Vol. 11, Pages 525: Transient Receptor Potential Mucolipin-1 Channels in Glioblastoma: Role in Patient’s Survival Cancers doi: 10.3390/cancers11040525 Authors: Maria Beatrice Morelli Consuelo Amantini Daniele Tomassoni Massimo Nabissi Antonella Arcella Giorgio Santoni A link between mucolipin channels and tumors has been recently suggested. Herein, we aim to investigate the transient receptor potential mucolipin (TRPML)-1 relevance in glioblastoma. The expression of this channel was evaluated via qRT-PCR and immunohistochemistry in biopsies from 66 glioblastoma patients and two human glioblastoma cell lines and compared to normal human brain, astrocytes, and epileptic tissues. The subcellular distribution of TRPML-1 was examined via confocal microscopy in the glioma cell lines. Then, to assess the role of TRPML-1, cell viability assays have been conducted in T98 and U251 cell lines treated with the specific TRPML-1 agonist, MK6-83. We found that MK6-83 reduced cell viability and induced caspase-3-dependent apoptosis. Indeed, the TRPML-1 silencing or the blockage of TRPML-1 dependent [Ca2+]i release abrogated these effects. In addition, exposure of glioma cells to the reactive oxygen species (ROS) inducer, carbonyl cyanide m-chlorophenylhydrazone (CCCP), stimulated a TRPML-1-dependent autophagic cell death, as demonstrated by the ability of the autophagic inhibitor bafilomycin A, the TRPML-1 inhibitor sphingomyelin, and the TRPML-1 silencing to com...
Source: Cancers - Category: Cancer & Oncology Authors: Tags: Article Source Type: research