Bronchial epithelial cell extracellular vesicles ameliorate epithelial-mesenchymal transition in COPD pathogenesis by alleviating M2 macrophage polarization

Publication date: Available online 11 April 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Shengyang He, Duanni Chen, Mengyun Hu, Li Zhang, Caihong Liu, Daniela Traini, Georges E. Grau, Zhengpeng Zeng, Junjuan Lu, Guanzhi Zhou, Lihua Xie, Shenghua SunAbstractChronic obstructive pulmonary disease (COPD) is partly characterized as epithelial-mesenchymal transition(EMT)-related airflow limitation. Extracellular vesicles(EVs) play crucial roles in the crosstalk between cells, affecting many diseases including COPD. Up to now, the roles of EVs in COPD are still debated. As we found in this investigation, COPD patients have higher miR-21 level in total serum EVs. EMT occurs in lungs of COPD mice. Furthermore, bronchial epithelial cells(BEAS-2B) could generate EVs with less miR-21 when treated with cigarette smoke extract (CSE), impacting less on the M2-directed macrophage polarization than the control-EVs(PBS-treated) according to EVs miR-21 level. Furthermore, the EMT processes in BEAS-2B cells were enhanced with the M2 macrophages proportion when co-cultured. Collectively, these results demonstrate that CSE-treated BEAS-2B cells could alleviate M2 macrophages polarization by modulated EVs, and eventually relieve the EMT process of BEAS-2B cells themselves under COPD pathogenesis, revealing a novel compensatory role of them in COPD.Graphical AbstractCigarette smoking was previously proved to be able to promote the M2 macrophage polarization in lungs of CO...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - Category: Nanotechnology Source Type: research