Genotypic and Phenotypic Characterization of Clinical Escherichia coli Sequence Type 405 Carrying IncN2 Plasmid Harboring blaNDM-1

Conclusion In this study, a self-transmissible IncN2 plasmid bearing blaNDM was detected in ST405 E. coli, which is a globally disseminated lineage. We highlight the dissemination potential of IncN2 plasmids harboring blaNDM. The presence of blaNDM mediated by plasmids in the high-risk clone ST405 E. coli linked with multiple virulence genes and resistance genes is increasing, which may create health concerns. Author Contributions YJ and ZL designed the experiments. YH analyzed the data and wrote the manuscript. CS and XG prepared the table and figures. YB carried out the bacteria identification. Funding The study was supported by grants from Focus on Research and Development Plan in Shandong Province (2016GSF201078) and Jinan Science and Technology Development Plan (201805021). Conflict of Interest Statement The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. Acknowledgments We would like to thank Professor Chunxiao Wu for his technical support and continuous encouragement. We would also like to thank Editage (www.editage.com) for English language editing. Supplementary Material The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmicb.2019.00788/full#supplementary-material FIGURE S1 | S1-PFGE and Southern Blot patterns of clinical strain JN24 (A) and transconjugant J24 (B) M, ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research