Membrane Sphingolipids Regulate the Fitness and Antifungal Protein Susceptibility of Neurospora crassa

This study was financially supported by the Austrian Science Fund (FWF) with the projects FWF P25894-B20 and FWF I1644-B20 to FM and a MUI Start grant of the Medical University of Innsbruck to MAK. Conflict of Interest Statement The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. Acknowledgments We thank Doris Bratschun-Khan for technical support. Supplementary Material The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmicb.2019.00605/full#supplementary-material Abbreviations AMP, antimicrobial protein; BP, BODIPY FL-EDA; CAT, conidial anastomosis tubes; CL, cardiolipin; ESI, electrospray ionization; GlcCer, glucosylceramide; H2O2, hydrogen peroxide; HPLC, high-performance liquid chromatography; (LC-) MS/MS, (liquid chromatography) tandem mass spectrometry; LCB, long chain base; LUV, large unilamellar vesicle; MIC, minimal inhibitory concentration; MIPC, mannosylinositol phosphorylceramides; MS, mass spectrometry; PAF, Penicillium chrysogenum antifungal protein; PAFB, Penicillium chrysogenum antifungal protein B; PC, phosphatidylcholines; PE, phosphatidylethanolamines; PI, phosphatidylinositols; PT, phosphinothricin; ROS, reactive oxygen species; t-BOOH, tert-butylhydroperoxide. Footnotes ^ www.gimp.org ^ www.broadinstitute.org ^ www.candidagenome.org ^ https://blast.ncbi.nlm.nih...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research