Evolution of the human cold/menthol receptor, TRPM8

In this study, we investigate whether alternative transcription has been a driving force in the evolution of the human forms of the cold receptor TRPM8.We identified 33 TRPM8 alternative mRNAs (24 new sequences) and their associated protein isoforms in human tissues. Using comparative genomics, we described the evolution of the human TRPM8 sequences in eight ancestors since the origin of Amniota, and estimated in which ancestors the new TRPM8 variants originated. In order to validate the estimated origins of this receptor, we performed experimental validations of predicted exons in mouse tissues. Our results suggest a first diversification event of the cold receptor in the Boreoeutheria ancestor, and a subsequent divergence at the origin of Simiiformes.Graphical abstract
Source: Molecular Phylogenetics and Evolution - Category: Molecular Biology Source Type: research