The Study of Dried Ginger and Linggan Wuwei Jiangxin Decoction Treatment of Cold Asthma Rats Using GC –MS Based Metabolomics

Conclusion In this study, serum and urine metabolites identified by GC–MS and general pharmacodynamic evaluation (morphological observation, histopathology, inflammatory factors, and visceral indices) were used to compare the effects of GJ and LGWWJX decoctions on cold asthma. Significant metabolic abnormalities were observed for 37 metabolites (15 in serum, 22 in urine) in rats with cold asthma. These altered metabolites might be potential biomarkers or therapeutic targets during development of cold asthma. Furthermore, pathway analysis demonstrated that pathways associated with energy metabolism and oxidative stress were disrupted in rats with cold asthma. GJ and LGWWJX regulated metabolites associated with various pathways. We demonstrated that the CM prescription LGWWJX exerted stronger effects than the single herb, GJ, at the metabolite level. However, the specific mechanisms of compatibility of these Chinese Medicines requires further study. Author Contributions YQH, XW, and YH conceived and designed the experiments. SR, FS, and YS performed the experiments. SR and YQH drafted this manuscript and analyzed the data. YH and XW provided final approval of the version to be published. All of the authors discussed the complete dataset to establish an integral and coherent analysis. Funding This work was supported by Anhui Province Natural Science Foundation (1708085MH196) and the National Natural Science Foundation of China (No. 81102811). Conflict of Interest St...
Source: Frontiers in Pharmacology - Category: Drugs & Pharmacology Source Type: research