Antimicrobial Blue Light Inactivation of Polymicrobial Biofilms

Antimicrobial Blue Light Inactivation of Polymicrobial Biofilms Raquel Ferrer-Espada1,2, Xiaojing Liu1,2, Xueping Sharon Goh1,2 and Tianhong Dai1,2* 1Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States 2Vaccine &Immunotherapy Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States Polymicrobial biofilms, in which mixed microbial species are present, play a significant role in persistent infections. Furthermore, polymicrobial biofilms promote antibiotic resistance by allowing interspecies transfer of antibiotic resistance genes. In the present study, we investigated the effectiveness of antimicrobial blue light (aBL; 405 nm), an innovative non-antibiotic approach, for the inactivation of polymicrobial biofilms. Dual-species biofilms with Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus (MRSA) as well as with P. aeruginosa and Candida albicans were reproducibly grown in 96-well microtiter plates or in the CDC biofilm reactor for 24 or 48 h. The effectiveness of aBL inactivation of polymicrobial biofilms was determined through colony forming assay and compared with that of monomicrobial biofilms of each species. aBL-induced morphological changes of biofilms were analyzed with confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). For 24-h old monomicrobial biofilms formed in 96-well microtiter plates, 6.30-log10 CFU inac...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research

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In this study, we evaluated the antimicrobial activity of the extracts from herbal plant Persecaria pensylvanicum’s flower, leaf, stem and root. This plant is also known by a common name Pennsylvania smartweed. The antimicrobial effect of the extracts was tested using minimimum inhibitory concentration (MIC) and minimum bacteriocidal concentration (MBC) methods in comparison with tetracycline, a commercial antibiotic.ResultsWe found that extract from P. pensylvanicum kills bovine mammary glands pathogenic S. aureus strain Newbould 305. The minimum inhibitory concentration (MIC) breakpoints for extracts from flower, l...
Source: European Journal of Integrative Medicine - Category: Complementary Medicine Source Type: research
Conclusion: The decision-to-delivery interval was ≤ 15 min in most women, suggesting that optimised organisation ensures short decision-to-delivery interval independently of the anaesthetic technique performed. As general anaesthesia was associated with worse neonatal outcomes, our results support the early insertion of an epidural catheter whenever there is any potential concern that an emergency caesarean section may be required.
Source: Anaesthesia, Critical Care and Pain Medicine - Category: Anesthesiology Source Type: research
AbstractIn this research, the copper hexacyanoferrate/SBA-15 composite was prepared by embedding method, which was further characterized using scanning electron microscope, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, Brunauer –Emmett–Teller, transmission electron microscopy and X-ray diffraction. The effects of different factors such as pH, temperature, contact time, initial cesium concentration on adsorption were studied. The pH significantly influenced the adsorption. The pseudo second-order kinetic model fitted th e adsorption well, indicating chemisorption. The Langmuir isothe...
Source: Journal of Radioanalytical and Nuclear Chemistry - Category: Nuclear Medicine Source Type: research
Publication date: Available online 24 May 2019Source: Accident Analysis &PreventionAuthor(s): Guibing Li, Fang Wang, Dietmar Otte, Ciaran SimmsAbstractHead injury is one of the most common injury types in vehicle-to-pedestrian collisions, which leads to death and long-term disabilities. However, detailed analysis of pedestrian head injuries in real world collisions is scarce. Thus the current study used two samples of 120 cases and 184 cases extracted from 1060 pedestrian collision cases captured during 2000–2015 from the GIDAS (German In-Depth-Accident Study) database to investigate the detailed characteristics ...
Source: Accident Analysis and Prevention - Category: Accident Prevention Source Type: research
Publication date: Available online 23 May 2019Source: Materials Science and Engineering: AAuthor(s): A. Chidambaram, S. Balasivanandha Prabu, K.A. PadmanabhanAbstractAluminum alloy 6061–5wt. %TiB2 in-situ metal matrix composite was produced by a salt-melt reaction between halide salts K2TiF6 and KBF4 inside AA6061 melt. The composites prepared were subjected to equal channel angular pressing (ECAP) up to 10 passes at ∼300 °C (0.45 Tm of AA6061). The improvement in strength and Vickers hardness due to grain refinement and redistribution of in-situ TiB2 particles was studied. The AA6061 (without TiB2) was als...
Source: Materials Science and Engineering: A - Category: Materials Science Source Type: research
Publication date: Available online 29 April 2019Source: Materials Research BulletinAuthor(s): Atsuo Kamura, Masahiko Ozaki, Naokazu Idota, Yoshiyuki SugaharaAbstractMagnetite (Fe3O4) nanoparticles (NPs) modified with n-dodecylphosphonic acid (DDPA) were prepared by one-pot nonaqueous synthesis using a salt comprising tetrachloroferrate (III) anions and methyltrioctylammonium cations as an Fe source, n-octylamine as a reductant, pyridine-N-oxide as an oxygen donor and DDPA as a phosphorus coupling reagent. The X-ray diffraction patterns, X-ray photoelectron spectroscopy and high-resolution transmission electron microscope (...
Source: Materials Research Bulletin - Category: Materials Science Source Type: research
Publication date: Available online 23 May 2019Source: Materials LettersAuthor(s): Ramkumar Chandran, Akhya Kumar Behera, Archana MallikAbstractIn the present work, we report on the electrodeposition of CuIn1-xGaxSe2 by a novel two-step deposition process. Initially, near stoichiometric CuInSe2 thin films of [Cu/In] ratio ∼ 1.15 was electrodeposited by single step technique using citric acid-sodium dodecyl sulfate (SDS) electrolyte. Further, the Ga-Se thin film was deposited potentiostatically in tartrate electrolyte to obtain a CISe/Ga-Se bilayer stack. The annealed CISe, CIGSe layers were subjected to XRD, FE-SEM and ...
Source: Materials Letters - Category: Materials Science Source Type: research
Publication date: Available online 23 May 2019Source: Materials Chemistry and PhysicsAuthor(s): Vinod Hegde, C.S. Dwaraka Viswanath, K.K. Mahato, Sudha D. KamathAbstract:Present work reports the colour tunable characteristics of Dy3+- doped zinc sodium bismuth borate glasses with Eu3+ and Pr3+ co-dopants synthesized through melt quench technique. The absence of sharp peaks in the XRD confirmed the amorphous nature of co-doped glasses. Absorption peaks of Dy3+, Eu3+ and Pr3+ions are found in visible and NIR region of the spectrum. The optical band gap of the glasses were determined through absorption spectra. The excitation...
Source: Materials Chemistry and Physics - Category: Materials Science Source Type: research
Publication date: Available online 24 May 2019Source: Journal of Membrane ScienceAuthor(s): Liang Shen, Ming Yi, Lian Tian, Fangqian Wang, Chun Ding, Shipeng Sun, Ang Lu, Li Su, Yan WangAbstractMembrane-based forward osmosis (FO) technology has attracted increasing interest in the field of water treatment due to its high economical and separation efficiency. However, the availability of a FO membrane with superior separation and antifouling properties is still the critical issue for its real application in wastewater purification. In this work, novel thin-film composite (TFC) membranes are developed by surface ionization w...
Source: Journal of Membrane Science - Category: Materials Science Source Type: research
Publication date: Available online 23 May 2019Source: Journal of Materials Research and TechnologyAuthor(s): An Huang, Honglian Dai, Xiaopei Wu, Zheng Zhao, Yanzeng WuAbstractThe mesoporous hydroxyapatite (HAP) powder was synthesized by using the microemulsion (hexadecyltrimethyl ammonium bromide (CTAB)/cyclohexane/n-octyl alcohol) system. The optimal ratios of concentrations of CTAB, cyclohexane and n-octyl alcohol in the microemulsion on morphology of the HAP were studied. The effects of pHs and calcination temperatures on the phase composition of the HAP powder were discussed. The phase composition, morphology, mesoporo...
Source: Journal of Materials Research and Technology - Category: Materials Science Source Type: research
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