A Short-Term High-Fat Diet Alters Glutathione Levels and IL-6 Gene Expression in Oxidative Skeletal Muscles of Young Rats

This study was carried out in strict accordance with recommendations of the National Institutes of Health guide for the care and use of Laboratory animals. Before undergoing the experimental work, the protocol was approved by the Comité Institutionnel de Protection des Animaux (CIPA) of UQAM (Permit Number: 0515-R3–759-0516). After a 3-day acclimatization period at UQAM’s animal facility, young (100–125 g; approximately 4 weeks old) male Wistar rats (Charles River, St-Constant, QC, Canada) were randomly fed with a regular chow diet (RCD; n = 13) or HFD (n = 12) for 14 days and submitted to a 12-h light/dark cycle starting at 06:00. Animals were given ad libitum access to the diets and water throughout the experimental protocol. Sacrifice was achieved under anesthesia (3% isoflurane at 0.5 L/min of O2) after a 4 h fast to standardize the feeding status of each animal. The soleus (SOL) muscle of both legs was collected for glutathione determination and RNA extraction. Diets Physiological fuel values were calculated from modified Atwater factors (3.5 kcal/g carbohydrate; 3.5 kcal/g protein; 8.5 kcal/g fat). The high fat diet was prepared from purified food-grade reagents according to a commercial formulation (D12492 diet, Research Diets Inc., New Brunswick, NJ, USA). It had a macronutrient weight content of 26.3% carbohydrate (19.2% kcal), 26.2% protein (19% kcal), and 34.9% fat (61.8%&nb...
Source: Frontiers in Physiology - Category: Physiology Source Type: research

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Source: SafetyLit - Category: International Medicine & Public Health Tags: Age: Adolescents Source Type: news
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We report the results of RXR ChIP-Seq on 2 month old wildtype and liver specific PPARg knockout mice, fed control-diet or high fat-diet supplemented with Pio or vehicle for 12 weeks
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ConclusionsInterventions for anxiety disorders in the perinatal period could be tailored to address anxieties about mother-infant relationship and co-morbid depressive symptoms.
Source: Journal of Anxiety Disorders - Category: Psychiatry Source Type: research
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Source: The Annals of Thoracic Surgery - Category: Cardiovascular & Thoracic Surgery Source Type: research
Authors: Saunders A, Messer LH, Forlenza GP Abstract Introduction: Automated insulin delivery for people with type 1 diabetes has been a major goal in the diabetes technology field for many years. While a fully automated system has not yet been accomplished, the MiniMed™ 670G artificial pancreas (AP) system is the first commercially available insulin pump that automates basal insulin delivery, while still requiring user input for insulin boluses. Determining the safety and efficacy of this system is essential to the development of future devices striving for more automation. Areas Covered: This review will pr...
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Source: Food Research International - Category: Food Science Source Type: research
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Source: Food Research International - Category: Food Science Source Type: research
In conclusion, the effect of reducing salt content is highly dependent on the cheese type. This factor should thus be taken into account by the dairy industry when the reduction of salt content is being considered. Moreover, the quality of raw products, in particular, the level of spoiler microorganisms, must be controlled before use during dairy processes.Graphical abstract
Source: Food Research International - Category: Food Science Source Type: research
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