Genetic code of WWI soldier's cholera mapped

(Wellcome Trust Sanger Institute) The oldest publicly available strain of the cholera-causing bacterial species, Vibrio cholerae, has had its genetic code read for the first time. The bacterium was isolated from a British soldier during World War One (WWI) and stored for over 100 years before being revived and sequenced. The results show that this strain is a unique, non-toxigenic strain of V. cholerae that's distantly related to the strains of bacteria causing cholera pandemics today and in the past.
Source: EurekAlert! - Infectious and Emerging Diseases - Category: Infectious Diseases Source Type: news

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ConclusionsWGS results allowed for mapping of short reads from APW-enriched specimen and culture isolate spotted filter papers this provided valuable molecular epidemiological sequence information onV.cholerae strains from remote, low-resource settings. These results identified the presence of co-infecting pathogens while providing rare insight into the specificV.cholerae strains causing outbreaks in cholera-endemic areas.
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
Toxigenic Vibrio cholerae serogroup O1 is the causative pathogen in the sixth and seventh cholera pandemics. Cholera toxin is the major virulent factor but other virulence and virulence-related factors play certa...
Source: BMC Genomics - Category: Genetics & Stem Cells Authors: Tags: Research article Source Type: research
This study reports the occurrence of multidrug resistant V. cholerae O1 in Lake Victoria that are genetically closely related to recent pandemic strains in Tanzania, Kenya and Uganda. The strains identified are also closely related to older pandemic strains recovered in the Democratic Republic of Congo, Burundi and Tanzania up until 1993, suggesting a long-term persistence and wide spatial distribution of pandemic strains within the region with the lake serving as a reservoir. These environmental isolates likely emerged from previous cholera outbreaks and survived in the lake environment for decades through various relatio...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
This study gives unprecedented insight into the physiology of VBNC cells and the drastically altered presence of their metabolic and structural proteins. Introduction Changes in the physical and chemical properties of their environment, such as heat, cold and salt stress, oxygen and nutrient deprivation, desiccation and changes in osmolarity, threaten the survival of bacteria, and they have therefore evolved various strategies to evade detrimental effects (Merchant and Helmann, 2012; Alvarez-Ordóñez et al., 2015; Guan et al., 2017). The facultative human pathogen Vibrio cholerae, is common in brackish a...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Conclusion The aim of our study was to determine if STs and virulence gene profiles of clinical and environmental V. cholerae non-O1, non-O139 strains originating from German coastal waters may be correlated and could be usable for a risk assessment of individual strains. The result of this study reveals that – given on the current knowledge about the potential pathogenicity of these strains – no such correlation is found. The MLST shows a high diversity of the bacteria, whereas a basic equipment with virulence genes is very similar between all strains. Due to horizontal gene transfer, in some strains the numb...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Genomic insights into the 2016–2017 cholera epidemic in YemenGenomic insights into the 2016–2017 cholera epidemic in Yemen, Published online: 02 January 2019; doi:10.1038/s41586-018-0818-3Isolates of the Vibrio cholerae O1 serotype Ogawa from Yemen are from a single sublineage of the seventh pandemic El Tor (7PET) lineage and are susceptible to several commonly used antibiotics as well as to polymyxins.
Source: Nature AOP - Category: Research Authors: Source Type: research
The Vibrio cholerae O1 serogroup is responsible for pandemic cholera and is divided into the classical and El Tor biotypes. Classical V. cholerae produces acid when using glucose as a carbon source, whereas El Tor V. cholerae produces the neutral product acetoin when using glucose as a carbon source. An earlier study demonstrated that Escherichia coli strains that metabolize glucose to acidic by-products drastically reduced the survival of V. cholerae strains in vitro. In the present study, zebrafish were fed 1% glucose and either inoculated with single V. cholerae or E. coli strains or coinfected with both V. cholerae and...
Source: Infection and Immunity - Category: Infectious Diseases Authors: Tags: Bacterial Infections Source Type: research
This study carried out extensive molecular characterization of CTXФ and ToxT regulon in ctx+ve strains of V. mimicus isolated from the Bengal coast. Southern hybridization, PCR, and DNA sequencing of virulence related-genes revealed the presence of an El Tor type CTX prophage (CTXET) carrying a novel ctxAB, tandem copies of environmental type pre-CTX prophage (pre-CTXEnv), and RS1 elements, which were organized in an array of RS1-CTXET-RS1-pre-CTXEnv-pre-CTXEnv Additionally, a novel variant of tcpA and toxT respectively, showing phylogenetic lineage to a clade of V. cholerae non-O1/O139, was identified. The V. mimicus str...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research
(Natural News) Cholera is dubbed as the first “global pandemic,” and for good reason — the disease has spawned seven pandemics around the world, and is still considered a threat to public health, according to the World Health Organization (WHO). The disease is known to be damaging once it hits, killing people “within hours” if not...
Source: NaturalNews.com - Category: Consumer Health News Source Type: news
[The Herald] A pall has been cast over Zimbabwe as the cholera pandemic has ravaged Harare's suburbs with the grim prospects that the outbreak could spread, claiming more lives.
Source: AllAfrica News: Health and Medicine - Category: African Health Source Type: news
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