Microarray Based Functional Analysis of Myricetin and Proteomic Study on Its Anti-Inflammatory Property.

Microarray Based Functional Analysis of Myricetin and Proteomic Study on Its Anti-Inflammatory Property. Biomed Res Int. 2019;2019:3746326 Authors: Li T, Zhu J, Deng F, Wu W, Zheng Z, Lv C, Li Y, Xiang W, Lu X, Qin S Abstract Myricetin has been reported as a promising chemopreventive compound with multiple biofunctions. To evaluate its influence on gene expressions in genome-wide set and further investigate its anti-inflammatory property, the present study performed Gene Ontology and Ingenuity Pathway Analysis (IPA) to describe the basic gene expression characteristics by myricetin treatment in HepG2 cells, confirmed its multi-biofunction by real-time fluorescent quantitative PCR (RT-qPCR), and further verified its anti-inflammatory property by Western blotting and bio-plex-based cytokines assay. The IPA data showed that 337 gene expressions (48% of the top molecules) are disturbed over 2-fold, and the most possible biofunctions of myricetin are the effect on "cardiovascular disease, metabolic disease, and lipid metabolism," via regulation of 28 molecules with statistic score of 46. RT-qPCR data confirmed the accuracy of microarray data, and cytokines assay results indicated that 6 of the total 27 inflammatory cytokine secretions were significantly inhibited by myricetin pretreatment, including TNF-α, IFN-γ, IL-1α, IL-1β, IL-2, and IL-6. The present study is the first time to elucidate the multi-function of myricetin in genome-wi...
Source: Biomed Res - Category: Research Authors: Tags: Biomed Res Int Source Type: research