BET Bromodomain Inhibitor iBET151 Impedes Human ILC2 Activation and Prevents Experimental Allergic Lung Inflammation

This study was supported by grants from GSK and the UK Medical Research Council (U105178805). Conflict of Interest Statement AM has grant funding from GSK and AstraZeneca/MedImmune. MB, DJ, AP, DT, and AvO are employees of GSK. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. Acknowledgments We are grateful to the Ares staff, genotyping facility, and flow cytometry core for their technical assistance. We thank Jen Walker for advice on the manuscript. Supplementary Material The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fimmu.2019.00678/full#supplementary-material Supplementary Figure S1. Human blood ILC subsets analyzed by flow cytometry. Supplementary Figure S2. RNAseq analysis of iBET151-modulated genes expressed by ex vivo human ILC2s. (A) List of the 270 differentially expressed genes between IL-33-stimulated hILC2 and IL-33-stimulated iBET (125 nM)-treated hILC2 from heatmap in Figure 1. (B) List of immune system process-associated genes increased by iBET151 following ex vivo culture of ILC2s. Supplementary Figure S3. Gene ontology analysis of iBET151-modulated genes expressed by in vitro-cultured human ILC2s. (A) List of the 204 differentially expressed genes between IL-33-stimulated hILC2 and IL-33-stimulated iBET (125 nM)-treated hILC2 from heatmap in Figure 2. ...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research

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