Actinobacteria Isolated From Laminaria ochroleuca: A Source of New Bioactive Compounds

Actinobacteria Isolated From Laminaria ochroleuca: A Source of New Bioactive Compounds Mariana Girão1,2, Inês Ribeiro1, Tiago Ribeiro1, Isabel C. Azevedo1, Filipe Pereira1, Ralph Urbatzka1, Pedro N. Leão1 and Maria F. Carvalho1* 1Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Porto, Portugal 2Department of Biology, Faculty of Sciences, University of Porto, Porto, Portugal Nature is the major reservoir of biologically active molecules. The urgent need of finding novel molecules for pharmaceutical application is prompting the research of underexplored environments, such as marine ecosystems. Here, we investigated cultivable actinobacteria associated with the macroalgae Laminaria ochroleuca and assessed their potential to produce compounds with antimicrobial or anticancer activities. A specimen of L. ochroleuca was collected in a rocky shore in northern Portugal, and fragments of tissues from different parts of the macroalgae (holdfast, stipe, and blades) were surface sterilized and plated in three culture media selective for actinobacteria. A total of 90 actinobacterial strains were isolated, most of which affiliated with the genus Streptomyces. Isolates associated with the genera Isoptericola, Rhodococcus, Nonomuraeae, Nocardiopsis, Microbispora, and Microbacterium were also obtained. Organic extracts from the isolates were tested for their antimicrobial activity using the agar-based disk diffusion method, foll...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research

Related Links:

Publication date: Available online 25 May 2019Source: Antiviral ResearchAuthor(s): R. Farrukee, J. Butler, P.C. Reading, A.C. HurtAbstract:Avian A(H7N9) infections in humans have been reported in China since 2013 and are of public health concern due to their severity and pandemic potential. Oseltamivir and peramivir are neuraminidase inhibitors (NAIs) routinely used for the treatment of A(H7N9) infections, but variants with reduced sensitivity to these drugs can emerge in patients during treatment. Zanamivir and laninamivir are NAIs that are used less frequently. Herein, we performed in vitro serial passaging experiments w...
Source: Antiviral Therapy - Category: Virology Source Type: research
In this study, we found that the PPL length of Korean SG isolates varied from 11 to 21 prolines and was longer than that of a live vaccine strain, SG 9R (9 prolines). According to growth competition in chickens, the growth of an SG isolate with a PPL length of 17 prolines exceeded that of an SG isolate with a PPL length of 15 prolines. We investigated the pseudogenes of the field isolates, SG 9R and reference strains in GenBank by resequencing and comparative genomics. The pseudogene profiles of the field isolates were notably different from those of the foreign SG strains, and they were subdivided into 7 pseudogene subgro...
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
In this study, in vivo and in vitro survivability and immunogenicity of four coliphages (TM1, TM2, TM3 and TM4) were investigated in naive chickens and chickens pre-immunised with phage TM1. Furthermore, two phages that displayed different survivability and immunogenicity (TM1 and TM3) were compared with respect to their efficacy in treating naive or pre-immunised (TM1) chickens suffering from colibacillosis. The efficacy of the treatments was evaluated based on body weight, relative organ weights, mortality, E. coli counts in the lungs as well as severity and frequency of internal organ lesions. At the end of the experime...
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
Publication date: Available online 24 May 2019Source: Veterinary MicrobiologyAuthor(s): Sante Roperto, Valeria Russo, Francesca De Falco, Alessandra Rosati, Cornel Catoi, Franco RopertoAbstractE5 protein, the major oncoprotein of the bovine Deltapapillomavirus genus, has been detected in 17 of the 19 urothelial cancers by molecular and morphological procedures. In 10 urothelial cancers, the oxygen sensitive subunit HIF-1α, which is upregulated by hypoxia, was overexpressed. Mitophagy, the selective autophagic removal of dysfunctional mitochondria, was upregulated in hypoxic neoplastic cells infected by BPVs which was...
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
Publication date: Available online 25 May 2019Source: Preventive Veterinary MedicineAuthor(s): Asseged Dibaba
Source: Preventive Veterinary Medicine - Category: Veterinary Research Source Type: research
Publication date: Available online 25 May 2019Source: Journal of Physics and Chemistry of SolidsAuthor(s): D. Alburquenque, L. Troncoso, J.C. Denardin, J.F. Marco, J.L. GautierAbstractManganese nickel oxides with a spinel-related structure, NixMn3-xO4-δ (x = 0.5 or 0.75), were synthesized by an ultrasound-assisted sol-gel method. Inductively coupled plasma atomic optical emission spectroscopy and energy-dispersive X-ray spectroscopy analysis showed good agreement with the nominal oxide compositions. The Rietveld refinement of the X-ray data indicated that both Ni0.5Mn2.5O4-δ and Ni0.75Mn2.25O4-δ cryst...
Source: Journal of Physics and Chemistry of Solids - Category: Physics Source Type: research
In conclusion, the compound has a protective effect against ALF in rats, and an underlying mechanism is involved in the TGF-β1/Smad signaling pathway. PMID: 31118972 [PubMed]
Source: Evidence-based Complementary and Alternative Medicine - Category: Complementary Medicine Tags: Evid Based Complement Alternat Med Source Type: research
Authors: Vigna L, Morelli F, Agnelli GM, Napolitano F, Ratto D, Occhinegro A, Di Iorio C, Savino E, Girometta C, Brandalise F, Rossi P Abstract Epidemiological data indicate that subjects affected by obesity have an increased risk of developing mood disorders. The relationship between obesity and mood disorders is bidirectional. We assessed whether a Hericium erinaceus treatment improved depression, anxiety, sleep, and binge eating disorders after 8 weeks of supplementation in subjects affected by overweight or obesity under a low calorie diet regimen. Looking for a possible clinical biomarker, we assessed the seru...
Source: Evidence-based Complementary and Alternative Medicine - Category: Complementary Medicine Tags: Evid Based Complement Alternat Med Source Type: research
Conclusion: Formal consensus was achieved for the first time on a core list of knowledge items on how exercises and physical activities might stimulate anticancer immunity. This core list might be considered at the time of developing training/educational interventions and/or continuing education for primary healthcare providers and CAM practitioners. Future studies are still needed to investigate if such consensual lists might improve congruence in cancer care continuum and improve survival rates and wellbeing of cancer patients and survivors. PMID: 31118968 [PubMed]
Source: Evidence-based Complementary and Alternative Medicine - Category: Complementary Medicine Tags: Evid Based Complement Alternat Med Source Type: research
Conclusions: WZYZ alleviates oligoasthenozoospermia by restoring the reproductive hormones and targeting the TGf-β1/Smads pathway. PMID: 31118967 [PubMed]
Source: Evidence-based Complementary and Alternative Medicine - Category: Complementary Medicine Tags: Evid Based Complement Alternat Med Source Type: research
More News: Academia | Austria Health | Biology | Biotechnology | Brain | Brain Cancers | Breast Carcinoma | Cancer | Cancer & Oncology | Cancer Therapy | Candida | Carcinoma | Chemistry | Chile Health | China Health | Clinical Trials | Databases & Libraries | Dermatology | Ductal Carcinoma | Egypt Health | Environmental Health | Epidemiology | France Health | Gastroenteritis | Genetics | Germany Health | Grants | Herbs | India Health | Infectious Diseases | Italy Health | Kelp | Laboratory Medicine | Learning | Lymphoma | Men | Microbiology | Molecular Biology | MRSA | Multidrug Resistance | Myeloma | Neuroblastoma | Neurology | Neuroscience | New Zealand Health | Nutrition | Nystatin | Organic | Penicillin | PET Scan | Portugal Health | Reproduction Medicine | Salmonella | Staphylococcus Aureus | Statistics | Study | Superbugs | Toxicology | Tuberculosis | UK Health | Universities & Medical Training | USA Health