Actinobacteria Isolated From Laminaria ochroleuca: A Source of New Bioactive Compounds

Conclusion This work adds to the knowledge available on actinobacteria associated with marine macroalgae and their bioactive potential. Our data indicates that the kelp forest alga L. ochroleuca is a valuable source for the isolation of actinobacteria, both in terms of abundance and diversity, and a reservoir of NP with antimicrobial and anticancer bioactivities. Author Contributions MG, PL, and MC designed and oversaw the whole study. MG and IR carried out the isolation of actinobacteria and taxonomic identification. MG and TR carried out cell line cytotoxicity assays. IA performed the kelp sampling. FP oversaw the molecular biology methodologies and performed the phylogenetic analysis together with MG. RU oversaw cell line assays and performed the statistical analysis. MG carried out the antimicrobial assays, LC-MS, and molecular networking/dereplication. MG and MC led the writing of the manuscript with contributions from all the co-authors. Funding This work was financed by national funds through Fundação para a Ciência e a Tecnologia (FCT) within the scope of the project IF/00791/2013/CP1197/CT0002, and by the strategic funding UID/Multi/04423/2019 through national funds provided by FCT and ERDF in the framework of the program PT2020. The authors wish to acknowledge grants (IF/00791/2013 to MC and IF/01358/2014 to PL) from the IFCT program supported by FCT, Fundo Social Europeu and Programa Operacional Potencial Humano. RU was supported by a post...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research