Wheat powdery mildew resistance gene Pm64 derived from wild emmer (Triticum turgidum var. dicoccoides) is tightly linked in repulsion with stripe rust resistance gene Yr5

Publication date: Available online 8 April 2019Source: The Crop JournalAuthor(s): Deyun Zhang, Keyun Zhu, Lingli Dong, Yong Liang, Genqiao Li, Tilin Fang, Guanghao Guo, Qiuhong Wu, Jingzhong Xie, Yongxing Chen, Ping Lu, Miaomiao Li, Huaizhi Zhang, Zhenzhong Wang, Yan Zhang, Qixin Sun, Zhiyong LiuAbstractStripe rust and powdery mildew are both devastating diseases for durum and common wheat. Pyramiding of genes conferring resistance to one or more diseases in a single cultivar is an important breeding approach to provide broader spectra of resistances in wheat improvement. A new powdery mildew resistance gene originating from wild emmer (Triticum turgidum var. dicoccoides) backcrossed into common wheat (T. aestivum) line WE35 was identified. It conferred an intermediate level of resistance to Blumeria graminis f. sp. tritici isolate E09 at the seedling stage and a high level of resistance at the adult plant stage. Genetic analysis showed that the powdery mildew resistance in WE35 was controlled by a dominant gene designated Pm64. Bulked segregant analysis (BSA) and molecular mapping indicated that Pm64 was located in chromosome bin 2BL4-0.50–0.89. Polymorphic markers were developed from the corresponding genomic regions of Chinese Spring wheat and wild emmer accession Zavitan to delimit Pm64 to a 0.55 cM genetic interval between markers WGGBH1364 and WGGBH612, corresponding to a 15 Mb genomic region on Chinese Spring and Zavitan 2BL, respectively. The genetic linkage map...
Source: The Crop Journal - Category: Food Science Source Type: research