Evaluation of human dental pulp stem cells behavior on a novel nanobiocomposite scaffold prepared for regenerative endodontics.

In this study, it was aimed to develop and characterize boron (B) modified bioactive glass nanoparticles (BG-NPs) containing cellulose acetate/oxidized pullulan/gelatin (CA/ox-PULL/GEL) three dimensional scaffolds with tubular morphology for dentin regeneration. 3D nanobiocomposite structures were prepared by thermally induced phase separation and porogen leaching methods and characterized by in vitro degradation analysis, water absorption (WA) capacity measurement, SEM, in vitro biomineralization analysis, porosity measurement and mechanical tests. Scaffolds lost about (30-40)% of their weight during one month and WA capacity decreased with increase in immersion time in phosphate buffer solution (PBS) during one month. According to SEM, aligned and tubular structures were formed with mean diameter of about 11 μm, and BG-NPS were distributed evenly in all parts of the scaffolds. Scaffolds (without BG-NPs) possessed the highest porosity percentage. Addition of 10% BG-NPs improved the mechanical properties of scaffolds. Scaffolds surfaces were fully covered by calcium phosphate (Ca-P) deposits after conditioning in simulated body fluid for 14 days with higher quantity of deposition in groups with inclusion of B-BG-NPs. Human dental pulp stem cells (hDPSCs) were isolated from third molar teeth and used in cell culture studies. In all groups, cells adhered well 1 day after culture. Group B14-10 showed a slight increase of proliferation than group (without BG-NPs) after 7...
Source: Appl Human Sci - Category: Physiology Authors: Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

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ConclusionsThe mean cost of a child avoiding any dental pain and/or infection (incidence) was £330 and the mean cost per episode of dental pain and/or infection avoided was £130. At these thresholds B + P has the highest probability of being considered cost-effective. Over the willingness to pay thresholds considered, the probability of B + P being considered cost-effective nev er exceeded 75%.
Source: Dental Technology Blog - Category: Dentistry Source Type: news
Abstract Human dentine is a mineralised dental tissue that consists of dentinal tubules surrounded by two distinct dentinal phases: peritubular dentine (PTD) and intertubular dentine (ITD). Dental caries, which manifests itself as a consequence of demineralisation, is one of the most common chronic diseases that affect the function of human teeth. Due to the difference in the packing density of crystallites, PTD and ITD exhibit different reaction rates to acid dissolution. The present study evaluates how the effective Young's modulus degrades and how the effective stress redistributes in demineralised human dentin...
Source: Journal of the Mechanical Behavior of Biomedical Materials - Category: Materials Science Authors: Tags: J Mech Behav Biomed Mater Source Type: research
Publication date: December 2020Source: Japanese Dental Science Review, Volume 56, Issue 1Author(s): Yumiko Kawashita, Sakiko Soutome, Masahiro Umeda, Toshiyuki SaitoSummaryRadiotherapy, often with concomitant chemotherapy, has a significant role in the management of head and neck cancer, however, radiotherapy induces adverse events include oral mucositis, hyposalivation, loss of taste, dental caries, osteoradionecrosis, and trismus, all of which have an impact on patients’ quality of life. Therefore, it is necessary to implement oral management strategies prior to the initiation of radiotherapy in patients with head ...
Source: Japanese Dental Science Review - Category: Dentistry Source Type: research
Publication date: Available online 19 February 2020Source: Journal of Evidence Based Dental PracticeAuthor(s): Alex Júnio Silva da Cruz, Johana Alejandra Moreno-Drada, Jacqueline Silva Santos, Mauro Henrique Nogueira Guimaraes de Abreu
Source: Journal of Evidence Based Dental Practice - Category: Dentistry Source Type: research
Publication date: Available online 18 February 2020Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Anne Marie Uwitonze, Sayeeda Rahman, Nkemcho Ojeh, William B. Grant, Harleen Kaur, Afrozul Haq, Mohammed S. RazzaqueAbstractAdequate nutrition is essential for maintaining good oral health. Minerals such as magnesium, calcium, and phosphorus found in the diet constitute the main structural components of the tooth. Their inadequacy leads to absorption impairment, increased bleeding tendency, bone resorption, looseness, and premature tooth loss. Inadequacy of those essential minerals is associated wi...
Source: The Journal of Steroid Biochemistry and Molecular Biology - Category: Biochemistry Source Type: research
Authors: DENTAL SUPPLEMENT, Mummolo S, Sapio S, Falco A, Vittorini OL, Quinzi V Abstract The aim of this study is to evaluate the success of functional rehabilitation of one dental session in patients with severe disabilities such as severe autism, baby bottle syndrome and severe odontophobia, and to assess how patient compliance pre- and post-intervention changes in moderate sedation. Between the beginning of 2016 and the end of 2018, 20 pedodontic patients with severe odontophobia, severe autism and baby bottle syndrome who needed dental treatment came to our attention. Patients were aged between 3 and 12 years, ...
Source: Journal of Biological Regulators and Homeostatic Agents - Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research
Conclusions: Knowledge gaps existed among surveyed Canadian health professionals on topics related to sugars consumption and the WHO sugars guideline. Future research should focus on tools to support better communication of sugars guideline and consistent use of sugars terminology. PMID: 32072816 [PubMed - as supplied by publisher]
Source: Canadian Journal of Dietetic Practice and Research - Category: Nutrition Authors: Tags: Can J Diet Pract Res Source Type: research
ConclusionThe findings of this study indicate that ECC prevalence and severity among this group of rural Cambodian children were high. Oral health programs focusing ECC prevention from an early age should be conducted in this population.
Source: Pediatric Dental Journal - Category: Dentistry Source Type: research
AbstractFluoride is an essential trace element for humans, and its deficiency or excess in the environment could lead to disease. To investigate the spatial distribution and health risk assessment of fluoride (F−) in drinking water, 302 tap water samples from Chongqing urban areas, China, were collected to analyze F− using an ion chromatograph. The results showed that (1) F− concentration in drinking water ranged from 0.100 to 0.503  mg/L, with an average of 0.238 ± 0.045 mg/L. (2) The spatial autocorrelation analysis showed that high–low clusters were mostly located i...
Source: Environmental Geochemistry and Health - Category: Environmental Health Source Type: research
Abstract Environmental lead (Pb) exposure can induce dysbacteriosis, impair oral health, and is associated with the development of dental caries. However, the mechanism is unclear. The aim of this study was to explore the effects of Pb toxicity on oral antimicrobial activity in children in an e-waste area. Results showed higher blood Pb levels in e-waste-exposed group children, accompanied by decreased saliva SAG (salivary agglutinin) concentrations, increased peripheral WBC (white blood cell) counts and monocyte counts, and elevated peripheral monocyte percentage. LnPb (natural logarithmic transformation of blood...
Source: Environmental Science and Pollution Research International - Category: Environmental Health Authors: Tags: Environ Sci Pollut Res Int Source Type: research
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