Asymmetric spontaneous intercalation of lutein into a phospholipid bilayer, a computational study

Publication date: Available online 6 April 2019Source: Computational and Structural Biotechnology JournalAuthor(s): Krzysztof Makuch, Michal Markiewicz, Marta Pasenkiewicz-GierulaAbstractLutein, a hydroxylated carotenoid, is a pigment synthesised by plants and bacteria. Animals are unable to synthesise lutein nevertheless, it is present in animal tissues, where its only source is dietary intake. Both in plants and animals, carotenoids are associated mainly with membranes where they carry out important physiological functions. Their trafficking to and insertion into membranes are not well recognised due to experimental difficulties. In this paper, a computational approach is used to elucidate details of the dynamics and energetics of lutein intercalation from the water to the phospholipid bilayer phase. The dynamics is studied using molecular dynamics simulation, and the energetics using umbrella sampling. Lutein spontaneous insertion into the bilayer and translocation across it proceed via formation of hydrogen bonds between its hydroxyl groups and water and/or phospholipid oxygen atoms as well as desolvation of its polyene chain. As lutein molecule is asymmetric, its bilayer intercalation is also asymmetric. The course of events and timescale of the intercalation are different from those of helical peptides. The time of full lutein intercalation ranges from 20 to 100 ns and its final orientation is predominately vertical. Nevertheless, some lutein molecules are in the fina...
Source: Computational and Structural Biotechnology Journal - Category: Biotechnology Source Type: research

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Authors: Han BH, Yoon JJ, Kim HY, Ahn YM, Jin SN, Wen JF, Lee HS, Lee YJ, Kang DG Abstract The proliferation of vascular smooth muscle cells plays a crucial role in pathogenesis of cardiovascular disease. The principal objective of this study was to determine the effects of Ojeoksan (OJS) on human aortic smooth muscle cell (HASMC) proliferation induced by tumor necrosis factor α (TNF-aα). Thymidine incorporation after TNF-α treatment was increased and this effect was inhibited significantly by OJS treatment. HASMC proliferation and migration by kinetic live cell imaging were also reduced by treatm...
Source: Journal of Physiology and Pharmacology - Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research
Publication date: Available online 22 August 2019Source: CellAuthor(s): Danyang Gong, Xinghong Dai, Jonathan Jih, Yun-Tao Liu, Guo-Qiang Bi, Ren Sun, Z. Hong ZhouSummaryAssembly of Kaposi’s sarcoma-associated herpesvirus (KSHV) begins at a bacteriophage-like portal complex that nucleates formation of an icosahedral capsid with capsid-associated tegument complexes (CATCs) and facilitates translocation of an ∼150-kb dsDNA genome, followed by acquisition of a pleomorphic tegument and envelope. Because of deviation from icosahedral symmetry, KSHV portal and tegument structures have largely been obscured in previ...
Source: Cell - Category: Cytology Source Type: research
In this study, we examined the anti-inflammatory property of an ethanol fraction (EEF6) fromR. minima root on lipopolysaccharide (LPS)-induced RAW 264.7 cells, as well as its underlying mechanism. The compound composition of EEF6 was determined by high-performance liquid chromatography-mass spectrometry. The result showed that five flavonoids compounds, 2 ′,4′,5,7-tetrahydroxyisoflavone, genistein-8-C-glucopyranoside, tricin, genistein, and daidzein, were identified in EEF6. In addition, EEF6 exhibited potent anti-inflammatory ability against LPS-stimulated RAW 264.7 cells via MAPK/NF- κB signaling pathwa...
Source: Inflammopharmacology - Category: Drugs & Pharmacology Source Type: research
Dalton Trans., 2019, Advance Article DOI: 10.1039/C9DT03073E, CommunicationMitsuki Yamashita, Shinnosuke Horiuchi, Koji Yamamoto, Tetsuro Murahashi The Pd2-translocation on a tetraene ligand is switchable between the thermodynamic equilibrium state and the photo-stationary state. The Pd7-translocation on a carotene ligand proceeds smoothly under photo-irradiation. To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Dalton Trans. latest articles - Category: Chemistry Authors: Source Type: research
ConclusionGEN inhibits IL-6–induced VEGF expression and angiogenesis partially through the JAK2/STAT3 pathway in RA, which has provided a novel insight into the antiangiogenic activity of GEN in RA.The translational potential of this articleOur study provides scientific guidance for the clinical translational research of GEN in the RA treatment.
Source: Journal of Orthopaedic Translation - Category: Orthopaedics Source Type: research
Publication date: Available online 23 August 2019Source: Cancer GeneticsAuthor(s): Chun Hang Au, Dona N. Ho, Beca B.K. Ip, Thomas S.K. Wan, Margaret H.L. Ng, Edmond K.W. Chiu, Tsun Leung Chan, Edmond S.K. MaAbstractDetection of chromosomal translocation is a key component in diagnosis and management of acute myeloid leukemia (AML). Targeted RNA next-generation sequencing (NGS) is emerging as a powerful and clinically practical tool, but it depends on expression of RNA transcript from the underlying DNA translocation. Here, we show the clinical utility of nanopore long-read sequencing in rapidly detecting DNA translocation ...
Source: Cancer Genetics - Category: Cancer & Oncology Source Type: research
AbstractA balanced robertsonian translocation (rob) results from fusion of 2 acrocentric chromosomes. Carriers are phenotypically normal and are often diagnosed because of recurrent miscarriages, infertility, or aneuploid offspring. Mortality and site-specific cancer risks in carriers have not been prospectively investigated. We followed 1,987 carriers diagnosed in Great Britain for deaths and cancer risk, over an average of 24.1 years. Standardized mortality and incidence ratios were calculated comparing the number of observed events against population rates. Overall mortality was higher for carriers diagnosed before age ...
Source: American Journal of Epidemiology - Category: Epidemiology Source Type: research
CONCLUSION: Low expression of TET2 in children with ALL is associated with poor prognosis and can be used as a molecular prognostic marker for risk group stratification. PMID: 31441600 [PubMed - as supplied by publisher]
Source: International Journal of Laboratory Hematology - Category: Hematology Authors: Tags: Int J Lab Hematol Source Type: research
In conclusion, our results show that S. pneumoniae directly inhibits purinergic signaling by inducing P2Y2 phosphorylation and internalization, resulting in the suppression of the calcium response of alveolar epithelial cells to ATP, thereby affecting cellular integrity and function.
Source: Journal of Biological Chemistry - Category: Chemistry Authors: Tags: Microbiology Source Type: research
Detection of chromosomal translocation is clinically important for disease diagnosis, prognostication, therapeutic target identification, and monitoring of hematological malignancies. Conventional detection methods are cytogenetics, PCR and fluorescence in situ hybridization (FISH). Next-generation sequencing (NGS) is emerging as a powerful new tool in the detection of chromosomal translocations, such as massively parallel RNA sequencing to detect recurrent gene fusions in leukemia [1]. In theory, whole genome sequencing (WGS) by NGS offers an unbiased approach for the identification of rearrangements, but is hitherto cons...
Source: Cancer Genetics and Cytogenetics - Category: Genetics & Stem Cells Authors: Tags: Short Communication Source Type: research
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