Dozens have fallen ill during a five-state E. Coli outbreak, and nobody knows where it’s coming from

The Centers for Disease Control and Prevention has yet to pinpoint the source to a specific food item, grocery store or restaurant chain.
Source: Washington Post: To Your Health - Category: Consumer Health News Authors: Source Type: news

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AbstractGiardia is a zoonotic gastrointestinal parasite responsible for a substantial global public health burden, and quantitative microbial risk assessment (QMRA) is often used to forecast and manage this burden. QMRA requires dose –response models to extrapolate available dose–response data, but the existing model forGiardia ignores valuable dose –response information, particularly data from several well‐documented waterborne outbreaks of giardiasis. The current study updatesGiardia dose –response modeling by synthesizing all available data from outbreaks and experimental studies using a Bayesi...
Source: Risk Analysis - Category: International Medicine & Public Health Authors: Tags: Original Research Article Source Type: research
This study was conducted to evaluate immunostimulation by Escherichia coli HSP70 (DnaK) in axenic European sea bass larvae in order to protect the larvae against vibriosis. DnaK stimulates the immune response in crustaceans and juvenile fish against bacterial infections. The use of axenic fish larvae allows to study immunostimulation in the absence of an interfering microbial community. At 7 days post-hatching, larvae received a single dose of alginate encapsulated recombinant DnaK. Two non-treated control groups in which animals either received empty alginate microparticles (C1) or no alginante microparticles (C2 and C3) ...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
l M Abstract Salmonella enterica is a foodborne pathogen often leading to gastroenteritis and is commonly acquired by consumption of contaminated food of animal origin. However, frequency of outbreaks linked to the consumption of fresh or minimally processed food of non-animal origin is increasing. New infection routes of S. enterica by vegetables, fruits, nuts and herbs have to be considered. This leads to special interest in S. enterica interactions with leafy products, e.g. salads, that are mainly consumed in minimally processed form. The attachment of S. enterica to salad is a crucial step in contamination, bu...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research
AbstractGiardia is a zoonotic gastrointestinal parasite responsible for a substantial global public health burden, and quantitative microbial risk assessment (QMRA) is often used to forecast and manage this burden. QMRA requires dose –response models to extrapolate available dose–response data, but the existing model forGiardia ignores valuable dose –response information, particularly data from several well‐documented waterborne outbreaks of giardiasis. The current study updatesGiardia dose –response modeling by synthesizing all available data from outbreaks and experimental studies using a Bayesi...
Source: Risk Analysis - Category: International Medicine & Public Health Authors: Tags: Original Research Article Source Type: research
K. G. Joensen et al.
Source: Emerging Infectious Diseases Journal - Category: Infectious Diseases Source Type: research
Cryptosporidium and Giardia are ubiquitous protozoan parasites that infect a broad range of vertebrate hosts, including domestic and wild animals as well as humans. Both parasites are of medical and veterinary importance. Infections with Cryptosporidium and Giardia in ruminants are associated with diarrhea outbreaks, mainly in young animals. Ruminants are potential sources of infection for humans because some species of Cryptosporidium and assemblages of Giardia duodenalis have been isolated from both ruminants and humans. Knowledge of these parasites has greatly expanded in the last 2 decades from simple microscopic obser...
Source: Veterinary Clinics of North America: Food Animal Practice - Category: Veterinary Research Authors: Source Type: research
(Flinders University) Salmonella is a key cause of foodborne gastroenteritis around the world, with most outbreaks linked to eggs, poultry meat, pork, beef, dairy, nuts and fresh produce. Now Flinders University researchers have found a simple solution for preventing salmonellosis affecting eggs through surface contamination, giving crucial help for food services industries.
Source: EurekAlert! - Infectious and Emerging Diseases - Category: Infectious Diseases Source Type: news
(Washington State University) Despite the perception that wild birds in farm fields can cause food-borne illness, a WSU study has found little evidence linking birds to E. coli, Salmonella and Campylobacter outbreaks.
Source: EurekAlert! - Biology - Category: Biology Source Type: news
In this study, we assessed propidium monoazide (PMA)-qPCR assays and novel methods combining PMA and loop-mediated isothermal amplification (LAMP) for the detection and quantification of VBNC E. coli O157:H7 and S. enterica in fresh produce. The performance of PMA-LAMP assays targeting wzy gene of E. coli O157:H7 and agfA gene of S. enterica and PMA-qPCR assays were compared in pure culture and spiked tomato, lettuce and spinach. No cross-reaction was observed in the specificity tests. The limit of detection (LOD) with PMA-LAMP was 9.0 CFU/reaction for E. coli O157:H7 and 4.6 CFU/reaction for S. enterica in pure culture, a...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research
Publication date: Available online 26 January 2020Source: Food ControlAuthor(s): Hanxu Pan, Kai Dong, Lei Rao, Liang Zhao, Xiaomeng Wu, Yongtao Wang, Xiaojun LiaoAbstractEscherichia coli O157:H7, the causative agent of haemorrhagic colitis and haemolytic uremic syndrome in humans, has been implicated in large food-borne outbreaks all over the world. When confronted with harsh environmental stresses, it can enter into a viable but nonculturable (VBNC) state, which poses a great risk to food safety and public health since conventional methods are invalid to detect VBNC cells. Herein, a system for detecting VBNC state Escheri...
Source: Food Control - Category: Food Science Source Type: research
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