CodonWizard – An intuitive software tool for customizable codon optimization in protein expression efforts with graphical user interface

Publication date: Available online 4 April 2019Source: Protein Expression and PurificationAuthor(s): Peter Rehbein, Jannik Berz, Patrick Kreisel, Harald SchwalbeAbstractOptimization of coding sequences to maximize protein expression yield is often outsourced to external service providers during oligonucleotide synthesis and thus remains a black box for many researchers. The presented software tool CodonWizard offers scientists a free, intuitive, easy-to-use software program for customized codon optimization. It provides features for analysis and completely customized modification/optimization of codon usage of any given input sequence data (protein/RNA/DNA) using combinatory algorithms. Contrary to comparable tools, this software may be used completely offline to protect intellectual property, but also provides means of sharing results between researchers: Complex codon optimization strategies may be planned, tested and archived easily due to the graphical user interface, providing a means for publicly sharing successful optimization approaches. The graphical user interface helps to reduce reluctance of scientist to use codon optimization hands on. As a benchmark, the reliability, intuitiveness and usefulness of the application could be demonstrated by increasing the yield of recombinant TEV-protease expressed in E. coli by several orders of magnitude after codon optimization using CodonWizard - Permanently available for download on the web at http://schwalbe.org.chemie.uni-f...
Source: Protein Expression and Purification - Category: Biochemistry Source Type: research