The Role of GM-CSF in a Mouse Model of Experimental Autoimmune prostatitis.

The Role of GM-CSF in a Mouse Model of Experimental Autoimmune prostatitis. Am J Physiol Renal Physiol. 2019 Apr 03;: Authors: Liu Y, Li Y, Liu Q, Wu Z, Cui J, Zhu K, Zhao H, Zhou C, Shi B Abstract The etiology of chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) is still unknown. Granulocyte macrophage colony-stimulating factor (GM-CSF) has been shown to play an important role in the development of autoimmune and inflammatory diseases. Here, we investigated the expression and function of GM-CSF in CP/CPPS patients and in a mouse model of Experimental Autoimmune prostatitis (EAP). GM-CSF mRNA levels were detected in expressed prostatic secretions samples from CP/CPPS patients and in prostate tissue from a mouse model of EAP. The expression of GM-CSF receptor in mouse prostate and dorsal root ganglia (DRG) were determined using PCR and immunohistochemistry. Behavioral testing and inflammation scoring were performed to evaluate the role of GM-CSF in disease development and symptom severity of EAP using GM-CSF knockout mice. The mRNA levels of putative nociceptive and inflammatory markers were measured in prostate after induction of EAP. Elevated GM-CSF mRNA levels were observed in expressed prostatic secretions samples from CP/CPPS patients compared with healthy volunteers. GM-CSF mRNA was also significantly increased in the prostate tissue of EAP mice model. The expression of GM-CSF receptors were confirmed in mouse prostate ...
Source: Am J Physiol Renal P... - Category: Urology & Nephrology Authors: Tags: Am J Physiol Renal Physiol Source Type: research