Sex-specific polymorphism of MET1 and ARR17 genes in Populus × sibirica.

Sex-specific polymorphism of MET1 and ARR17 genes in Populus × sibirica. Biochimie. 2019 Mar 29;: Authors: Melnikova NV, Kudryavtseva AV, Borkhert EV, Pushkova EN, Fedorova MS, Snezhkina AV, Krasnov GS, Dmitriev AA Abstract The genus Populus is an effective model in tree genetics. This genus includes dioecious species and, recently, whole genome resequencing of P. trichocarpa and P. balsamifera enabled the identification of sex-linked regions and sex-associated single nucleotide polymorphisms (SNPs). These results created new opportunities to study sex determination in poplars. In the present work, we performed deep sequencing of genes encoding METHYLTRANSFERASE1 (MET1) and homolog of ARABIDOPSIS RESPONSE REGULATOR 17 (ARR17), which are localized in a sex-linked region of Populus genome and contain a number of sex-associated SNPs. Amplicon libraries for 38 samples of P. × sibirica (19 males and 19 females) were sequenced on MiSeq Illumina (300 nt paired-end reads) and approximately 4000x coverage was obtained for each sample. In total, from 80 to 179 SNPs were detected in poplar individuals for MET1, and from 16 to 49 SNPs were detected for ARR17. We identified 17 sex-specific SNPs (11 in MET1 and 6 in ARR17) - they were present in all males but absent in all females. For identified sex-specific SNP sites, females were homozygous, while males were heterozygous. Moreover, colocation of sex-specific SNPs confirming the XY sex-determi...
Source: Biochimie - Category: Biochemistry Authors: Tags: Biochimie Source Type: research