Functional and structural characterization of IdnL7, an adenylation enzyme involved in incednine biosynthesis

Adenylation enzymes play an important role in the selective incorporation of the cognate carboxylate substrates in natural product biosynthesis. Here, the biochemical and structural characterization of the adenylation enzyme IdnL7, which is involved in the biosynthesis of the macrolactam polyketide antibiotic incednine, is reported. Biochemical analysis showed that IdnL7 selects and activates several small amino acids. The structure of IdnL7 in complex with an l-alanyl-adenylate intermediate mimic, 5 ′ -O-[N-(l-alanyl)sulfamoyl]adenosine, was determined at 2.1   Å resolution. The structure of IdnL7 explains the broad substrate specificity of IdnL7 towards small l-amino acids.
Source: Acta Crystallographica Section F - Category: Biochemistry Authors: Tags: macrolactam antibiotics polyketides crystal structure adenylation enzyme natural product biosynthesis research communications Source Type: research

Related Links:

This report continues the development of ALIs as a clinical tool in wildlife while systematically testing one possible method for determining an optimal ALI for a particular species. PMID: 32228119 [PubMed - as supplied by publisher]
Source: Stress - Category: Research Tags: Stress Source Type: research
Authors: Park J, Kim J, Chen Y, Song HC, Chen Y, Zheng M, Surh YJ, Kim UH, Park JW, Joe Y, Chung HT Abstract Oxidative stress is recognised as a key factor that can lead to cellular senescence and aging. Carbon monoxide (CO) is produced by haemoxygenase-1 (HO-1), which exerts cytoprotective effects in aging-related diseases, whereas the effect of CO on cellular senescence and aging has not been elucidated. In the current study, we clearly demonstrated that CO delays the process of cellular senescence and aging through regulation of miR-34a and Sirt1 expression. CO reduced H2O2-induced premature senescence in human ...
Source: Free Radical Research - Category: Research Tags: Free Radic Res Source Type: research
Publication date: Available online 1 April 2020Source: Journal of Microbiological MethodsAuthor(s): Wisnu Tafroji, Felicia Monica Bernadette, Ernawati A. Giri Rachman, Dodi Safari
Source: Journal of Microbiological Methods - Category: Microbiology Source Type: research
Publication date: Available online 2 April 2020Source: Journal of Microbiology, Immunology and InfectionAuthor(s): Chun-Min Kang, Xiang-Jun Chen, Ching-Chin Chih, Chen-Ching Hsu, Ping-Hung Chen, Tai fen Lee, Lee-Jene Teng, Po-Ren Hsueh
Source: Journal of Microbiology, Immunology and Infection - Category: Microbiology Source Type: research
Publication date: Available online 1 April 2020Source: Journal of Microbiology, Immunology and InfectionAuthor(s): Chien-Yu Lee, Pi-Sheng Wang, Yuan-Der Huang, Yung-Ching Lin, Yung-Nien Hsu, Shih-Chung Chen
Source: Journal of Microbiology, Immunology and Infection - Category: Microbiology Source Type: research
Publication date: Available online 1 April 2020Source: Journal of Microbiology, Immunology and InfectionAuthor(s): Chia-Hung Liao, Shih-Chang Hung, Yuan-Ti Lee, Hung-Chang Hung, Po-Ren Hsueh
Source: Journal of Microbiology, Immunology and Infection - Category: Microbiology Source Type: research
Publication date: Available online 2 April 2020Source: Statistics &Probability LettersAuthor(s): Junhao Guo, Jie Zhou, Sanfeng Hu
Source: Statistics and Probability Letters - Category: Statistics Source Type: research
Publication date: Available online 1 April 2020Source: Statistics &Probability LettersAuthor(s): S. Mousavinasr, C.R. Gonçalves, C.C.Y. Dorea
Source: Statistics and Probability Letters - Category: Statistics Source Type: research
Publication date: 1 May 2020Source: Sensors and Actuators A: Physical, Volume 306Author(s): Chuang Ge, Leixiang Bian, Jiayang Li, Mingyou Zhong, Songtong Han, Yunfei Jia
Source: Sensors and Actuators A: Physical - Category: Physics Source Type: research
Publication date: Available online 1 April 2020Source: Results in PhysicsAuthor(s): U.S. Okorie, A.N. Ikot, E.O. Chukwuocha, G.J. Rampho
Source: Results in Physics - Category: Physics Source Type: research
More News: Biochemistry