Metabolomic profile of young male goats seropositive to small ruminant lentivirus – a longitudinal study

Publication date: Available online 1 April 2019Source: Small Ruminant ResearchAuthor(s): Maria Milczarek, Michał Czopowicz, Olga Szaluś-Jordanow, Lucjan Witkowski, Tomasz Nalbert, Iwona Markowska-Daniel, Emilia Bagnicka, Ryszard Puchała, Iwona Kosieradzka, Jarosław KabaAbstractWe carried out a longitudinal study to determine the change of concentration of a large set of metabolites over the first 2 years of goats’ life and identify metabolites whose concentration follows different patterns in small ruminant lentivirus(SRLV)-infected and uninfected goats. Thirteen clinically healthy male goats, born to SRLV-infected does and castrated at the age of 4 months, were followed up for 2 years. They were serologically tested weekly and on this basis assigned to seropositive (n = 7) and seronegative (n = 6) group. At the age of 6, 12, 18 and 24 months their serum was subjected to metabolomic analysis using a commercial AbsoluteIDQ® p180 Kit assay designed to measure concentrations of 188 metabolites. Finally, 134 metabolites had concentrations above the limit of detection and were included in the analysis. Twenty three metabolites differed between seropositive and seronegative goats at a false discovery rate of 25% and with fold change of at least 1.5: 21 metabolites at the age of 6 months and 2 metabolites at the age of 24 months. Concentration of 96 metabolites tended to change significantly in time: concentration of 57 metabolites increased, 12 decreased, and 27 fluc...

Source: Small Ruminant Research - April 2, 2019 Category: Zoology Source Type: research