Repeated retinal photocoagulation in monkeys for optimization of laser-induced choroidal neovascularization model.

Repeated retinal photocoagulation in monkeys for optimization of laser-induced choroidal neovascularization model. Exp Eye Res. 2019 Mar 27;: Authors: Lin X, Wang Q, He M Abstract Laser-induced choroidal neovascularization (CNV) model in nonhuman primates has played a critical role in the development of new therapies for neovascular age-related macular degeneration. The widespread use of this model, however, has been limited by its high costs, mainly due to the lower efficiency of animal use. To address this problem, we optimized the CNV model by repeated photocoagulation in the same eye of each animal and preliminarily evaluated this model for assessment of an anti-vascular endothelial growth factor (VEGF) agent. Seven rhesus monkeys were included and divided into two groups, which were named the laser-only and laser-bevacizumab groups. Each animal underwent 3 retinal photocoagulation sessions in the same eye at 4-week intervals to induce CNV. The animals in the laser-bevacizumab group received treatment in both eyes during the first photocoagulation session. Three weeks after the first laser treatment, the animals in the laser-bevacizumab group were given an intravitreal injection of bevacizumab in the repeated photocoagulation eye, while no medicine was administered to the contralateral eye. To assess the severity and development of CNV produced by each laser treatment, fluorescein angiography (FA) was performed in all animals at ...
Source: Experimental Eye Research - Category: Opthalmology Authors: Tags: Exp Eye Res Source Type: research