Proteomics of Asrij perturbation in Drosophila lymph glands for identification of new regulators of hematopoiesis.

Proteomics of Asrij perturbation in Drosophila lymph glands for identification of new regulators of hematopoiesis. Mol Cell Proteomics. 2019 Mar 28;: Authors: Sinha S, Ray A, Abhilash L, Kumar M, Sreenivasamurthy SK, Keshava Prasad TS, Inamdar MS Abstract Hematopoiesis is the process of differentiation of precursor blood cells into mature blood cells that is controlled by a complex set of molecular interactions. Understanding hematopoiesis is important for the study of hematological disorders. However, a comprehensive understanding of how physiological and genetic mechanisms regulate blood cell precursor maintenance and differentiation is lacking. Owing to simplicity and ease of genetic analysis, the Drosophila melanogaster lymph gland (LG) is an excellent model to study hematopoiesis. Here, we quantitatively analyzed the LG proteome under genetic conditions that either maintain precursors or promote their differentiation in vivo, by perturbing expression of Asrij, a conserved endosomal regulator of hematopoiesis. Using iTRAQ-based quantitative proteomics, we determined the relative expression levels of proteins in Asrij-knockout and overexpressing LGs from 1,500 larval dissections in comparison to wild type. Our data showed that at least 6.5% of the Drosophila proteome is expressed in wild type LGs. Of the 2,133 proteins identified, 780 and 208 proteins were common to previously reported cardiac tube and hemolymph proteomes, respect...
Source: Molecular and Cellular Proteomics : MCP - Category: Molecular Biology Authors: Tags: Mol Cell Proteomics Source Type: research