Effects of size and geographical origin on Atlantic salmon, Salmo salar, mucin O-glycan repertoire.

Effects of size and geographical origin on Atlantic salmon, Salmo salar, mucin O-glycan repertoire. Mol Cell Proteomics. 2019 Mar 28;: Authors: Benktander J, Venkatakrishnan V, Padra JT, Sundh H, Sundell K, Murughan AVM, Maynard B, Linden SK Abstract Diseases cause ethical concerns and economic losses in the Salmonid industry. The mucus layer comprised of highly O-glycosylated mucins is the first contact between pathogens and fish. Mucin glycans govern pathogen adhesion, growth and virulence. The Atlantic salmon O-glycome from a single location has been characterized and the inter-individual variation was low. Since inter-individual variation is considered a population based defence, hindering the entire population from being wiped out by a single infection, low inter-individual variation among Atlantic salmon may be a concern. Here, we analysed the O-glycome of 25 Atlantic salmon from six cohorts grown under various conditions from Sweden, Norway and Australia (Tasmania) using mass spectrometry. This expanded the known Atlantic salmon O-glycome by 60% to 169 identified structures. The mucin O-glycosylation was relatively stable over time within a geographical region but the size of the fish affected skin mucin glycosylation. The skin mucin glycan repertoires from Swedish and Norwegian Atlantic salmon populations were closely related compared to Tasmanian ones, regardless of size and salinity, with differences in glycan size and comp...
Source: Molecular and Cellular Proteomics : MCP - Category: Molecular Biology Authors: Tags: Mol Cell Proteomics Source Type: research