Proteomics analysis of Trichoplusia ni midgut epithelial cell brush border membrane vesicles.

This study reports the identification of proteins from BBM vesicles (BBMVs) using single dimension polyacrylamide gel electrophoresis coupled with multi-dimensional protein identification technology. More than 3000 proteins were associated with the BBMV, of which 697 were predicted to possess either a signal peptide, at least one transmembrane domain or a GPI-anchor signal. Of these, bioinformatics analysis and manual curation predicted that 185 may be associated with the BBMV or epithelial cell plasma membrane. These are discussed with respect to their predicted functions, namely digestion, nutrient uptake, cell signaling, development, cell-cell interactions, and other functions. We believe this to be the most detailed proteomic analysis of the lepidopteran midgut epithelium membrane to date, which will provide information to better understand the biochemical, physiological and pathological processes taking place in the larval midgut.
PMID: 29064633 [PubMed - indexed for MEDLINE]

Source: Genomics Proteomics ... - March 29, 2019 Category: Genetics & Stem Cells Authors: Javed MA, Coutu C, Theilmann DA, Erlandson MA, Hegedus DD Tags: Insect Sci Source Type: research