Spatial and temporal studies of metabolic activity: Contrasting biochemical kinetics in tissues and pathways during fasted and fed states.

Spatial and temporal studies of metabolic activity: Contrasting biochemical kinetics in tissues and pathways during fasted and fed states. Am J Physiol Endocrinol Metab. 2019 Mar 26;: Authors: Daurio NA, Wang Y, Chen Y, Zhou H, Carballo-Jane E, Mane J, Rodriguez CG, Zafian P, Houghton A, Addona G, McLaren DG, Zhang R, Shyong BJ, Bateman KP, Downes DP, Webb M, Kelley DE, Previs SF Abstract The regulation of nutrient homeostasis, i.e. the ability to transition between fasted and fed states, is fundamental in maintaining health. Since food is typically consumed over limited (anabolic) periods, dietary components must be processed and stored to counterbalance the catabolic stress that occurs between meals. Herein, we contrast tissue- and pathway-specific metabolic activity in fasted and fed states. We demonstrate that knowledge of biochemical kinetics which is obtained from opposite ends of the energetic spectrum can allow mechanism-based differentiation of healthy and disease phenotypes. Rat models of Type 1 and Type 2 diabetes serve as case studies for probing spatial and temporal patterns of metabolic activity via 2H-water labeling. Experimental designs which capture integrative whole-body metabolism, including meal-induced substrate partitioning, can support an array of research surrounding metabolic disease; the relative simplicity of the approach that is discussed here should enable routine applications in preclinical models. ...
Source: American Journal of Physiology. Endocrinology and Metabolism - Category: Physiology Authors: Tags: Am J Physiol Endocrinol Metab Source Type: research