In vitro propagation and phytochemical assessment of Perovskia abrotanoides Karel. (Lamiaceae) – A medicinally important source of phenolic compounds

Publication date: Available online 24 March 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Shahla Ghaderi, Samad Nejad Ebrahimi, Hamid Ahadi, Sara Eslambolchi Moghadam, Mohammad Hossein MirjaliliAbstractPerovskia abrotanoides Karel. (Lamiaceae) is an aromatic herb that is mainly grown in the mountains of Northeastern Iran across Northern Pakistan and Northwestern India. Here, a suitable system for regenerating P. abrotanoides via direct organogenesis was performed for the first time. In vitro-grown shoot tips were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations (1.0, 2.0, and 3.0 mg L−1) of kinetin (KN) and 6-benzylaminopurine (BAP) alone or in combination with two concentrations (0.5 or 1.0 mg L−1) of 2,4-dichlorophenoxyacetic (2,4-D), α-naphthalene acetic acid (NAA), and indole-3-butyric acid (IBA). MS medium supplemented with 3 mg L−1 BAP and 0.5 mg L−1 IBA with shoot proliferation percentage (83.3%) and shoot-forming capacity (SFC) index (5.5) was the optimal medium for shoot induction in the plant. Rooting of elongated shoots was performed on half-strength MS medium fortified with different concentrations (0.1, 0.5, and 1.0 mg L−1) of IBA, 2,4-D, and NAA. The highest rooting percentage (100%) with a mean root number (5.9 ± 1.35) was obtained on the free-hormone medium. Rooted plantlets were successfully acclimatized in the greenhouse (75%) and transferred to natural conditions. ...
Source: Biocatalysis and Agricultural Biotechnology - Category: Biotechnology Source Type: research