Regulation of water ‐soluble glucan synthesis by the Streptococcus mutans dexA gene effects biofilm aggregation and cariogenic pathogenicity

In this study, we identify the role of theS mutans dexAgene in dextran ‐dependent aggregation in biofilm formation. Our results show that deletion ofdexA (SmudexA) results in increased transcription of EPS synthesis ‐related genes, includinggtfB, gtfD, andftf. Interestingly, we reveal that inactivating thedexA gene may lead to elevated WSG synthesis inS mutans , which results in dysregulated cariogenicity in vivo. Furthermore, structural analysis provides new insights regarding the lack of mannose monosaccharides, especially in the WSG synthesis of theSmudexA mutants. The biofilm phenotypes that are associated with the reduced glucose monosaccharide composition in both WSG and water ‐insoluble glucan shift the dental biofilm to reduce the cariogenic incidence of theSmudexA mutants. Taken together, these data reveal that EPS synthesis fine ‐tuning by thedexA gene results in a densely packed EPS matrix that may impede the glucose metabolism of WSG, thereby leading to the lack of an energy source for the bacteria. These results highlightdexA targeting as a potentially effective tool in dental caries management.
Source: Molecular Oral Microbiology - Category: Microbiology Authors: Tags: ORIGINAL ARTICLE Source Type: research

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Because I wanted a benign and healthy way for followers of the Wheat Belly lifestyle to recreate dishes such as chocolate chip cookies, cheesecake, and pies with none of the health problems of grains or sugars, I helped Wheat-Free Market develop its Virtue Sweetener  product. Yes, you could do without such sweeteners. But I learned long ago when I introduced Wheat Belly concepts to patients in my cardiology practice that having options while entertaining friends, during holidays, and pleasing kids was important for staying on course on this lifestyle. Before I understood how to use such natural sweeteners, patients wo...
Source: Wheat Belly Blog - Category: Cardiology Authors: Tags: Open blood sugar diabetes Dr. Davis Inflammation insulin low-carb monk fruit natural sweeteners undoctored virtue sweetener Weight Loss wheat Wheat Belly Total Health Source Type: blogs
CONCLUSIONS: DNI treatment on an emergency basis requires proper diagnosis and effective management. To confirm diagnosis and prevent serious complications, it is essential for physicians to recognize the spaces of the head and neck that are likely to be affected by DNI. PMID: 31814344 [PubMed - in process]
Source: The Israel Medical Association Journal - Category: General Medicine Tags: Isr Med Assoc J Source Type: research
Conclusions: Childhood caries in the rural area was far more severe than that in the urban area. This disparity was determined by several factors such as the high proportion of left-behind children in the rural area (e.g. children who remain in rural regions of China while their parents leave to work in urban areas). Our government should put the prevention of caries among rural children at a higher priority in the near future to narrow the gap between rural and urban areas.
Source: Oral health and Preventive dentistry - Category: Dentistry Source Type: research
The co-occurrence of caries and periodontitis and a possible association is still a matter of debate. Thus, the aim of the study was to determine the co-occurrence of caries and periodontitis in Chilean adults.
Source: BMC Oral Health - Category: Dentistry Authors: Tags: Research article Source Type: research
Dental problems are the most prevalent chronic disease worldwide, with up to half of all kindergarten children having tooth decay. However, there is a lack of evidence of whether unaddressed dental needs (UDNs...
Source: BMC Pediatrics - Category: Pediatrics Authors: Tags: Research article Source Type: research
International&American Associations for Dental Research Alexandria, VA, USA - 2019 marks the Centennial of theJournal of Dental Research (JDR). Over the last century theJDR has been dedicated to the dissemination of new knowledge and information on all sciences relevant to dentistry and to the oral cavity and associated structures in health and disease. To celebrate, theJDR is featuring a yearlong, commemorative article and podcast series that highlights topics that have transformed dental, oral and craniofacial research over the past 100 years. The importance and value of behavioral sciences in dentistry has long been...
Source: Dental Technology Blog - Category: Dentistry Source Type: news
We present the case of an adult patient with cherubism, who underwent dental extraction due to chronic apical periodontitis, at a bony site that was involved with a lesion in the affected jaw. A 19-year-old woman with cherubism, exhibiting severe bilateral expansion of the lower jaw, had a decayed second molar present in a lesion of her affected mandible. Although she had reached adulthood, it was unclear if active growth of the lesion had been arrested. On removal, the tooth showed osseous ankyloses and required root separation. Postoperative healing was normal and without complications, such as wound infection and regrow...
Source: Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology - Category: ENT & OMF Source Type: research
ConclusionHigh consumption of sports drinks is positively associated with erosive tooth wear. Children whose caregivers ’ educational level is low are less likely to present erosive tooth wear.
Source: European Archives of Paediatric Dentistry - Category: Dentistry Source Type: research
This article reports on the decayed, missing and filled teeth (DMFT) scores in relation to demographic characteristics for a mixed‐ancestry South African population.MethodsThis cross ‐sectional epidemiological study was part of the ongoing Cape Town Bellville South Vascular and Metabolic Health Study. Each participant signed an informed consent form prior to enrolling in the study. During a clinical examination, DMFT score and components were recorded for each participant. Dat a analysis was performed with the statistical Package STATA 15.ResultsA sample of 1,885 participants was included in the study. Sixty ‐eight p...
Source: International Dental Journal - Category: Dentistry Authors: Tags: Scientific Research Report Source Type: research
Publication date: Available online 4 December 2019Source: Journal of Herbal MedicineAuthor(s): Farkash Yosi, Feldman Mark, Ginsburg Isaac, Shalish Miriam, Steinberg DoronAbstractDental biofilm is recognized as the primary cause of oral infections, caries being the most common. Orthodontic appliances might increase the risk for dental caries and white spot lesions. Phytomedicinal methods were shown to inhibit dental biofilm formation. The goal of this study was to examine whether Padma hepaten (PH), a polyphenolic formula derived from traditional Tibetan medicine, could inhibit the caries-inducing properties of S. mutans. B...
Source: Journal of Herbal Medicine - Category: Complementary Medicine Source Type: research
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