Thermodynamic insights into interaction of protein coated gold nanoclusters with DNA and influence of coating on drug binding

Publication date: Available online 24 March 2019Source: Journal of Molecular LiquidsAuthor(s): Achal Mukhija, Nand KishoreAbstractBiocompatibility and ease of surface modification make gold nanoclusters potential to be used in nanomedicine and revolutionizing cancer therapy. Low solubility and bioavailability of drug limits the use of conventional drug administrative methods and hence the need for drug delivery vehicles arises. Different drug delivery vehicles have been used in the past but usually, the information is limited to qualitative in nature. Here, we have reported quantitative insights into interaction of human serum albumin coated gold nanoclusters with anticancer drugs cyclophosphamide, 5-fluorouracil and hydroxyurea, and further interaction of drug-nanoclusters complexes with deoxyribonucleic acid (DNA). Calorimetry, in combination with spectroscopy has been used to characterize HSA coated gold nanoclusters and to obtain thermodynamic parameters associated with interaction of drug with nanoclusters and drug-nanoclusters complex with DNA. Binding of drugs with HSA coated gold nanoclusters has been compared with binding of drugs with free HSA. We found, HSA coated gold nanocluster show better binding efficiency than free HSA due to the minor conformational changes in HSA upon coating. Binding of HSA coated gold nanoclusters has also been studied with DNA to understand the effect of nanocluster binding upon conformation of DNA. Thermodynamic signatures for interacti...
Source: Journal of Molecular Liquids - Category: Molecular Biology Source Type: research

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Conclusion: For lay people, risk information, including the statistical numeric part, is perceived as highly normatively charged, often as an emotionally significant threat. It seems necessary to provide lay people with a deeper understanding of risk information and of the limitations of genetic knowledge with respect to one’s own health responsibility. Introduction Technological progress over the recent years has led to an unprecedented increase in speed and precision of genetic testing. The number of inherited disorders and risk factors that can be detected through genetic testing is growing rapidly and gen...
Source: Frontiers in Genetics - Category: Genetics & Stem Cells Source Type: research
Notice NOT-DE-19-007 from the NIH Guide for Grants and Contracts
Source: NIH Funding Opportunities (Notices, PA, RFA) - Category: Research Source Type: funding
Publication date: 1 August 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 180Author(s): Jingwen Shi, Shuiping Liu, Yuan Yu, Changyu He, Lianjiang Tan, Yu-Mei ShenAbstractDevelopment of polymer-drug conjugate capable of controlled drug release is urgently needed for gastric cancer therapy. Herein, arginine-glycine-aspartic acid (RGD)-decorated polyethylene glycol (PEG)-paclitaxel (PTX) conjugates containing disulfide linkage were synthesized. The amphiphilic PEG-PTX conjugates were found to assemble into micelles (RGD@Micelles), which would be decomposed under the reduction of glutathione (GSH) and finally relea...
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research
Notice NOT-DE-19-006 from the NIH Guide for Grants and Contracts
Source: NIH Funding Opportunities (Notices, PA, RFA) - Category: Research Source Type: funding
The U.S. Food and Drug Administration approves a dozen cancer drugs every year, but the agency has greenlighted only six since 1978 specifically for childhood cancers. It’s not like adult cancers can always be repurposed for children at a lower dose. The causes of pediatric cancer are much different, and they generally have few mutations and occur when the normal development process is accentuated and inappropriately activated after birth. The National Cancer Institute estimated that more than…
Source: Health Care:Biotechnology headlines - Category: Biotechnology Authors: Source Type: news
In this report, we are adding an extra finding, in which we show that the levels of circulating NRP-1 were significantly increased in patients who received NAC and had a partial response. Assessing patients who underwent NAC indicated that the levels of NRP-1 measured post-NAC were significantly higher in younger patients and patients with either a low or a medium body mass index (BMI), as well as in patients who remained with larger tumor size and partial response. Previously, we showed that SNAI1 expression in the immune cells collected from the peripheral blood of breast cancer patients was significantly higher in patie...
Source: Frontiers in Oncology - Category: Cancer & Oncology Source Type: research
Conclusion Several TISC-based immunotherapeutic approaches are under development in various stages of preclinical studies. As outlined in this review article, a careful and more exhaustive genetic and metabolic understanding of TISC-associated phenotypes is critical to develop novel TISC based immunotherapies. Various components within the tumor microenvironment such as tumor cells, infiltrating immune cells, and supporting stromal cells impact the TISC metabolism. This unique metabolic profile leads to upregulation of certain enzymes and proteins such as ALDH1, CEP55, IDO COA1 etc., which can be utilized for development ...
Source: Frontiers in Oncology - Category: Cancer & Oncology Source Type: research
Publication date: 23 April 2019Source: Cell Reports, Volume 27, Issue 4Author(s): Erin E. Mulkearns-Hubert, Luke A. Torre-Healy, Daniel J. Silver, Jennifer T. Eurich, Defne Bayik, Emily Serbinowski, Masahiro Hitomi, John Zhou, Bartlomiej Przychodzen, Renliang Zhang, Samuel A. Sprowls, James S. Hale, Tyler J. Alban, Artem Berezovsky, Brent A. Bell, Paul R. Lockman, Babal K. Jha, Justin D. LathiaSummaryGap-junction-mediated cell-cell communication enables tumor cells to synchronize complex processes. We previously found that glioblastoma cancer stem cells (CSCs) express higher levels of the gap junction protein Cx46 compared...
Source: Cell Reports - Category: Cytology Source Type: research
Discussion: The data reviewed here show successful treatment on biologics despite concurrent malignancy, though confirmatory research is needed. J Drugs Dermatol. 2019;18(4):387-390. PMID: 31013012 [PubMed - as supplied by publisher]
Source: Journal of Drugs in Dermatology - Category: Dermatology Tags: J Drugs Dermatol Source Type: research
ACS Applied Materials&InterfacesDOI: 10.1021/acsami.9b03519
Source: ACS Applied Materials and Interfaces - Category: Materials Science Authors: Source Type: research
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