Improvement of in vitro potency assays by a resting step for clinical-grade chimeric antigen receptor engineered T cells

DiscussionCryopreservation is a challenge for the functional activity of CAR-T cells. However, CAR-T cells regain their potency by overnight incubation at 37°C, which mimics the clinical application setting. Therefore, an overnight resting step should be included in in vitro potency assays.
Source: Cytotherapy - Category: Cytology Source Type: research