Let ‑7a‑5p may participate in the pathogenesis of diabetic nephropathy through targeting HMGA2.

Let‑7a‑5p may participate in the pathogenesis of diabetic nephropathy through targeting HMGA2. Mol Med Rep. 2019 Mar 19;: Authors: Wang T, Zhu H, Yang S, Fei X Abstract Diabetic nephropathy (DN) is one of the most common complications of diabetes mellitus (DM), and has been demonstrated as one of the major causes of renal failure. In a previous study, it was noted that microRNA let‑7a‑5p was downregulated in DN; however, the underlying mechanism requires additional investigation. The aim of the present study was to investigate the roles of let‑7a‑5p in the pathogenesis of DN and its associated mechanism. The renal tissues of db/db and db/m mice, and renal mesangial cells treated with high concentrations of glucose were obtained; reverse transcription‑quantitative polymerase chain reaction, and western blot analysis were applied to detect the expression of let‑7a‑5p and high‑mobility group AT‑hook 2 (HMGA2) in vivo and in vitro. In addition, renal mesangial cells cultured under high‑glucose conditions (20 and 30 mmol/l) were transfected with either let‑7a‑5p mimics or let‑7a‑5p inhibitors. The effects of let‑7a‑5p on the proliferation and apoptosis of renal mesangial cells were examined using CCK‑8 and flow cytometry methods. Additionally, cells were collected and the expression of phosphoinositide 3‑kinase (PI3K), phosphorylated protein kinase B (p‑AKT) and HMGA2 was analyzed with western ...
Source: Molecular Medicine Reports - Category: Molecular Biology Tags: Mol Med Rep Source Type: research