Calcitonin gene related peptide gene-modified rat bone mesenchymal stem cells are effective seed cells in tissue engineering to repair skull defects.

Calcitonin gene related peptide gene-modified rat bone mesenchymal stem cells are effective seed cells in tissue engineering to repair skull defects. Histol Histopathol. 2019 Mar 21;:18102 Authors: Yu X, Liu S, Chen X, Du Y, Yin X, Du Y, Li S Abstract The study is to verify whether Calcitonin gene related peptide (CGRP) gene-modified rat bone mesenchymal stem cells (BMSCs) are promising seed cells with great potential to repair bone defect. A recombinant lentiviral vector overexpressing CGRP peptide, pLenO-DCE-CGRP, was constructed and transfected into rBMSCs to obtain stable CGRP expression. Differently transfected rBMSCs: CGRP- modified rBMSCs (CGRP group), empty vector- modified rBMSCs (Vector group) and normal rBMSCs (Control group) were transferred to collagen scaffold to repair rat skull defects (n=6). Skull specimens were obtained at 4 and 8 weeks after operation. Expressions of osteogenesis-related indexes OCN (osteocalcin) and BMP-2 (Bone morphogenetic protein-2) were detected by immunohistochemistry and western blotting. Histological observation, Masson trichrome staining and Micro-computed tomography (Micro-CT) were applied to evaluate defect repair. Tartrate-resistant acid phosphatase (TRAP) was applied to observe bone remodeling at 4 weeks. PGP9.5 was detected to observe nerve fibers formation at 8 weeks. Osteogenic markers OCN and BMP-2 were significantly higher in CGRP -modified BMSCs at 4 weeks, compared with the vect...
Source: Histology and Histopathology - Category: Cytology Tags: Histol Histopathol Source Type: research