Use of low-cost substrates for cost-effective production of extracellular and cell-bound lipases by a newly isolated yeast Dipodascus capitatus A4C

Publication date: Available online 21 March 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Khurshid Ahmed Baloch, Apichat Upaichit, Benjamas CheirsilpAbstractAs the enzyme production and its application is a costly process mainly because of the inducible production, this study aimed to screen the basic components of media for the production of cost-effective lipases. A newly isolated yeast strain, namely Dipodascus capitatus A4C is a specific yeast that could produce lipases in both extracellular and cell-bound forms. Various low-cost substrates were applied as single or mixed carbon sources for D. capitatus A4C. These included non-edible vegetable oil (Jatropha curcas oil), crude glycerol, and molasses. The suitable cost-effective carbon source for extracellular lipase production was 2% J. curcas oil, which gave high extracellular lipases activity of 2,670 U/L at 96 h. While the suitable low-cost carbon source for cell-bound lipase production was 2% molasses, which gave high cell-bound lipase activity of 373 U/L at 48 h. The present work emphasized that the lipolytic activity of D. capitatus A4C can be improved with a compatible use of low-cost carbon sources as co-substrates.
Source: Biocatalysis and Agricultural Biotechnology - Category: Biotechnology Source Type: research

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Publication date: Available online 24 April 2019Source: Journal of Critical CareAuthor(s): Philippe Montravers, Ignacio Martin-Loeches
Source: Journal of Critical Care - Category: Gastroenterology Source Type: research
Publication date: Available online 25 April 2019Source: European Annals of Otorhinolaryngology, Head and Neck DiseasesAuthor(s): G. Dumas, H. Tan, L. Dumas, P. Perrin, A. Lion, S. SchmerberAbstractObjectiveTo establish optimum stimulus frequency and location of bone conducted vibration provoking a skull vibration induced nystagmus (SVIN) in superior semi-circular canal dehiscences.MethodsSVIN 3D components in 40 patients with semi-circular canal dehiscence (27 unilateral and 13 bilateral) were compared with a group of 18 patients with severe unilateral vestibular loss and a control group of 11 volunteers.ResultsIn unilater...
Source: European Annals of Otorhinolaryngology, Head and Neck Diseases - Category: ENT & OMF Source Type: research
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Source: Hormone Research in Paediatrics - Category: Endocrinology Source Type: research
Publication date: Available online 24 April 2019Source: The Lancet Diabetes &EndocrinologyAuthor(s): Stuart W Flint
Source: The Lancet Diabetes and Endocrinology - Category: Endocrinology Source Type: research
Publication date: Available online 24 April 2019Source: The Lancet Diabetes &EndocrinologyAuthor(s): Peter H Bennett
Source: The Lancet Diabetes and Endocrinology - Category: Endocrinology Source Type: research
Publication date: Available online 13 April 2019Source: Molecular and Cellular EndocrinologyAuthor(s): Matti Poutanen, Trevor M. Penning
Source: Molecular and Cellular Endocrinology - Category: Endocrinology Source Type: research
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Source: Diabetes and Metabolic Syndrome: Clinical Research and Reviews - Category: Endocrinology Source Type: research
ConclusionDiabetes was correlated significantly with dyslipidemia and atherogenic risk in parallel with an increase in IFN-γ production and hematological inflammatory biomarkers; leukocytes, neutrophil/lymphocyte and platelet/lymphocyte ratios, MPV and PDW values. The amelioration in inflammatory biomarkers was associated with improvement in glycemic control.
Source: Diabetes and Metabolic Syndrome: Clinical Research and Reviews - Category: Endocrinology Source Type: research
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